Far-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit 9122 from ImmunoChemistry Technologies

Product Specs
ItemFar-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit
CompanyImmunoChemistry Technologies
Price $270.85Supplier Page View Company Product Page
Catalog Number9122
Quantity25-50 Tests
Detection TargetCaspase 1
ComponentsFLICA Caspase-1 Reagent (660-YVAD-FMK), 1 vial, #6323 10X Cellular Wash Buffer, 15 mL, #6164 Fixative, 6 mL, #636 Kit Manual
Absorbance / Excitation660 nm
Emission690 nm
Sample TypeCell culture
Molecule Name660-VAD-FMK
TypeCell Viability

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ImmunoChemistry Technologies

P.O. Box 1560

Davis, CA 95617-1560

United States

Phone: (800) 824-8540

Email: orders@antibodiesinc.com

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Website: www.antibodiesinc.com/collections/immunochemistry-technologies

(1) TREM2 sustains glucose metabolic homeostasis to drive antibacterial defense during sepsisiScienceApril 17, 2026ZeHua Wu, XueKe Wang, YiChao Ren, LiHua Shen, YiHang Pan, Yan Zhang, JiaQian Fan, Qiang Shu, Xiangming Fang, QiXing Chen(NJTEN3, eBioscience). For caspase-1 activity assay, FLICA 660-YVAD-FMK caspase-1 detection probe (9122, ImmunoChemistry) was added in working solution (1:60) after surface staining and incubated at 37°C

(2) Palmitoylation of Tfr1 enhances platelet ferroptosis and liver injury in heat strokeActa Pharmaceutica Sinica BJanuary 1, 2026Qiyuan An, Riqing Wei, Zhicheng Huang, Youyong Tang, Minghao Wang, Sixiao He, Kaihua Huang, Zhifeng Liu, Meimei Zhang, Ru Li, et al.(113807, Biolegend, San Diego, CA, USA; 561939, BD Biosciences, Franklin Lakes, NJ, USA), Caspase-1 (97/9122, ImmunoChemistry, Bloomington, MN, USA), CD61 (104306, Biolegend), CD42b (303903, Biolegend), CD62p

(3) SMAC mimetics sensitize HIV-infected cells to oncolytic virus-mediated deathFront ImmunolDecember 18, 2025Bengisu Molyer, Yasmeen Ameeriar, Jonathan B. AngelFar-Red Fluorescent FLICA® 660 Caspase-3/7 (DEVD) Assay Kit (cat. no. 9125) and the Far-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit (cat. no. 9122; both from ImmunoChemistry Technologies, Davis, CA, USA) according to the manufacturer’s protocol. The

(4) A20 Attenuates Inflammatory Injury in Bovine Endometrial Epithelial Cells Through Autophagy-Mediated NLRP3 Inflammasome InactivationAnimals (Basel)December 5, 2025Yongshuai Jiang, Fan Fei, Xiaoyu Wang, Yeqi Jiang, Kangjun Liu, Long Guo, Luying Cui, Heng Wang, Junsheng Dong, Jianji Lifrom Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The FLICA 660 Caspase-1 assay kit (9122) was purchased from Immunochemistry Technologies (Davis, CA, USA). The Trizol reagent (DP424) was obtained

(5) Comparative Analysis of Septin Modifiers, Forchlorfenuron and UR214‐9, on Mitochondrial Fragmentation and Lytic Cell DeathCytoskeleton (Hoboken)July 30, 2025Dominik Brokatzky, Rajdeep Das, Hannah Painter, Rakesh K. Singh, Serge Mostowythrough 4‐mm strainers. Cells were then stained in 300 μL FLICA 660 Caspase‐1 (Immunochemistry, CAT#9122) detection reagent and LIVE/DEAD fixable violet stain (1:1000) for 1 h at RT in darkness. After PBS

(6) Caspase-1-licensed pyroptosis drives dsRNA-mediated necroptosis and dampens host defense against bacterial pneumoniaPLoS PathogMay 13, 2025Qinyu Luo, Lihua Shen, Shiyue Yang, Yan Zhang, Yihang Pan, Zehua Wu, Qiang Shu, Qixing ChenCD31 (BioLegend, 102509), FLICA 660-YVAD-FMK caspase-1 detection probes (ImmunoChemistry Technologies, 9122), and fixable viability dye 780 (BD Biosciences, 565388). Control isotype antibodies were used to determine

(7) Ghrelin Inhibits Inflammasomes Activation in Astrocytes, Alleviates Pyroptosis, and Prevents Lipopolysaccharide-induced Depression-like Behavior in MiceInflammationDecember 19, 2024Xiaoou Han, Xiying Fu, Wanxu Guo, Yaqi Liu, Jiangjin Sun, Tian Wang, Wei YangLabtech, Germany). Flow Cytometry The caspase-1 fluorescent inhibitor probe, FLICA 660-YVAD-FMK (ICT-9122, Immunochemistry), was used to assess primary astrocyte pyroptosis by double staining with PI (KGA214,

(8) Mechanics-activated fibroblasts promote pulmonary group 2 innate lymphoid cell plasticity propelling silicosis progressionNature communicationsNovember 12, 2024He Y, Yang F, Yang L, Yuan H, You Y, Chen Y, Wu X, Min H, Chen J, Li C.fibroblasts was examined using the FLICA660 caspase 1 assay kit (ImmunoChemistry Technologies, Cat: 9122). A FLICA 660 probe was used to detect cells expressing active caspase 1. Assays were performed by flow cytometry

(9) Bystander monocytic cells drive infection-independent NLRP3 inflammasome response to SARS-CoV-2mBioOctober 16, 2024Hsieh LL, Looney M, Figueroa A, Massaccesi G, Stavrakis G, Anaya EU, D'Alessio FR, Ordonez AA, Pekosz AS, DeFilippis VR, et al.a fluorescent caspase-1 inhibitor probe 660-YVAD-FMK, FLICA660, (ImmunoChemistry Technologies, cat# 9122) was added to supernatant at 24 h post-infection and incubated for 1 h at 37°C. Cells were washed, and

(10) S100A8/9 modulates perturbation and glycolysis of macrophages in allergic asthma micePeerJApril 18, 2024Xiaoyi Ji, Chunhua Nie, Yuan Yao, Yu Ma, Huafei Huang, Chuangli HaoUK). The gating strategy of BALF is described in Fig. S1. For pyroptosis, the FLICA 660 Caspase-1 (9122, ImmunoChemistry, Bloomington, MN, USA) and PI staining (556547, BD, Franklin Lakes, NJ, USA) were used

(11) Garlic oil supplementation blocks inflammatory pyroptosis-related acute lung injury by suppressing the NF-κB/NLRP3 signaling pathway via H(2)S generationAgingApril 12, 2024Dilxat T, Shi Q, Chen X, Liu X.Flow cytometry Caspase-1 activity, an indicator of pyroptosis, was measured using the FLICA 660 Caspase-1 Assay kit (cat. 9122, ImmunoChemistry, USA). First, fetal bovine serum (FBS)-washed and trypsin-digested cells were resuspended

(12) Low-Dose Chemotherapy Preferentially Shapes the Ileal Microbiome and Augments the Response to Immune Checkpoint Blockade by Activating AIM2 Inflammasome in Ileal Epithelial CellsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2024Pu C, Li Y, Fu Y, Yan Y, Tao S, Tang S, Gai X, Ding Z, Gan Z, Liu Y, et al.at 350 g, 4 °C for 5 min. For flow cytometry analysis, single cells were incubated with FLICA‐YVAD (#9122, ImmunoChemistry Technologies) for 1 h at 37 °C and re‐suspended every 20 min, followed by washing according

(13) The CARD8 inflammasome dictates HIV/SIV pathogenesis and disease progressionCellFebruary 29, 2024Wang Q, Clark KM, Tiwari R, Raju N, Tharp GK, Rogers J, Harris RA, Raveendran M, Bosinger SE, Burdo TH, et al.proteins","Chemicals, peptides, and recombinant proteins" FLICA660-Caspase 1 reagent,ImmunoChemistry Technologies,9122 APC Annexin V,BioLegend,640941 Propidium Iodide Solution,BioLegend,421301 Zombie Violet Fixable Viability,BioLegend,423114 Zombie

(14) Shigella Serotypes Associated With Carriage in Humans Establish Persistent Infection in ZebrafishThe Journal of Infectious DiseasesOctober 18, 2023Vincenzo Torraca, Dominik Brokatzky, Sydney L Miles, Charlotte E Chong, P Malaka De Silva, Stephen Baker, Claire Jenkins, Kathryn E Holt, Kate S Baker, Serge MostowyμL of staining solution for active caspase 1 (FLICA 660 Caspase-1 Assay Kit, probe 660-YVAD-FMK, No. 9122; ImmunoChemistry Technologies) prepared per the manufacturer's guidelines. Upon staining, cells were

(15) Downregulation of RCN1 promotes pyroptosis in acute myeloid leukemia cellsMolecular OncologySeptember 30, 2023Sisi Deng, Yuming Pan, Na An, Fengyi Chen, Huan Chen, Heng Wang, Xiaojing Xu, Rui Liu, Linlin Yang, Xiaomei Wang, et al.Myd88‐I, HY‐19744; MedChemExpress). Cleaved‐caspase‐1 was detected by the FLICA® 660 Caspase‐1 Assay Kit (9122; Immunochemistry, Bloomington, MN, USA), and cell death was tested by the Annexin V‐PE Apoptosis Detection

(16) Heme oxygenase‑1 inhibits renal tubular epithelial cell pyroptosis by regulating mitochondrial function through PINK1Experimental and therapeutic medicineMay 1, 2023Li HB, Mo YS, Zhang XZ, Zhou Q, Liang XD, Song JN, Hou LN, Wu JN, Guo Y, Feng DD, et al.and mixed gently with fluorescence-labeled Annexin V nucleic acid dye 660 Caspase-1 Assay (cat. no. 9122; ImmunoChemistry Technologies, LLC.). The Annexin V nucleic acid dye was placed in the dark at room

(17) Chemical inhibition of DPP9 sensitizes the CARD8 inflammasome in HIV-1-infected cellsNature chemical biologyApril 1, 2023Clark KM, Kim JG, Wang Q, Gao H, Presti RM, Shan L.Cayman Chemical. The FLICA660 Caspase1 staining reagents were purchased from ImmunoChemistry Technologies (#9122). The following antibodies were used in this study: Anti-CD3 (Biolegend #300333, clone: HIT3a, 1mg/mL),

(18) The protein kinase R modifies gut physiology to limit colitisFrontiers in ImmunologyFebruary 17, 2023Howard Chi Ho Yim, Arindam Chakrabarti, Sean Kessler, Hiroyuki Morimoto, Die Wang, Dhanya Sooraj, Afsar U. Ahmed, Carol de la Motte, Robert H. Silverman, Bryan RG. Williams, et al.immunoblot. Immune fluorescence Caspase-1 (Casp1) activity was assessed by FAM-FLICA and FLICA-660 (No. 97 and 9122, respectively, from ImmunoChemistry Technologies LLC or, alternatively, ThermoFisher Scientific). The

(19) Astragaloside IV Attenuates High-Glucose-Induced Impairment in Diabetic Nephropathy by Increasing Klotho Expression via the NF-κB/NLRP3 AxisJournal of diabetes researchJanuary 1, 2023He J, Cui J, Shi Y, Wang T, Xin J, Li Y, Shan X, Zhu Z, Gao Y.GGATGAAGGTGATGTCAGGTGC 2.15. Flow Cytometry Analysis for Cell Pyroptosis A FLICA 660 Caspase-1/PI Assay kit (9122; ImmunoChemistry Technologies, USA) was used to detect the pyroptosis rate of podocytes. Cells in six-well

(20) WTAP-mediated N6-methyladenosine modification of NLRP3 mRNA in kidney injury of diabetic nephropathyCellular & Molecular Biology LettersDecember 1, 2022Jianzi Lan, Bowen Xu, Xin Shi, Qi Pan, Qing Tao(FLICA 660 in vitro Active Caspase-1 Detection Kit, ImmunoChemistry Technologies, Bloomington, MN, USA; #9122) according to manufacturer’s instructions and with propidium iodide (PI; Invitrogen, Waltham, MA, USA;

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Far-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit from ImmunoChemistry Technologies

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Far-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit from ImmunoChemistry Technologies

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