GloMelt™ Thermal Shift Protein Stability Kit 33021-1 from Biotium, Inc.

Product Specs
ItemGloMelt™ Thermal Shift Protein Stability Kit
CompanyBiotium, Inc.
Price $529.00Supplier Page View Company Product Page
Catalog Number33021-1
Source/Expression SystemIgG from domesticated goat (Capra aegagrus hircus)
Sample TypeThermal shift assay, differential scanning fluorimetry
ApplicationsThermal shift assay, differential scanning fluorimetry
StorageProtect from light;

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Biotium, Inc.

46117 Landing Parkway

Fremont, CA 94538

United States

Phone: 510-265-1027
510-265-1352

Email: order@biotium.com

Company Profile

Website: https://biotium.com/company/

(1) Pathogenic variants in the polycystin pore helix cause distinct forms of channel dysfunctionProc Natl Acad Sci U S AJune 17, 2025Orhi Esarte Palomero, Eduardo Guadarrama, Paul G. DeCaenSEC purified PKD2 variants in A8-35 were mixed to a final concentration of 1X GloMelt dye (Biotium 33021-T) and 50 nM ROX dye internal standard in 20 µL of buffer (25 mM HEPES pH =7.4, 150 mM NaCl, and 1 mM

(2) A single amino acid in the Salmonella effector SarA/SteE triggers supraphysiological activation of STAT3 for anti-inflammatory gene expressionCell reportsApril 22, 2025Gaggioli MR, Jones AG, Panagi I, Washington EJ, Loney RE, Muench JH, Foster MW, Brennan RG, Thurston TLM, Ko DC.the vehicle control. Subsequently, the STAT3 and peptide mixtures were added to 1X Glomelt (Biotium #33021-1, Fremont, California) a dye that binds hydrophobic residues and fluoresces upon protein denaturation

(3) Regulation of urea cycle by reversible high-stoichiometry lysine succinylationNature MetabolismMarch 6, 2024Ran Zhang, Jingqi Fang, Xueshu Xie, Chris Carrico, Jesse G. Meyer, Lei Wei, Joanna Bons, Jacob Rose, Rebeccah Riley, Ryan Kwok, et al.proteins, differential scanning fluorimetry using a thermal shift protein stability kit (Biotium, cat. no. 33021) according to the manufacturer’s instructions. The enzymes were heated from 25.0 °C to 99.0 °C with

(4) A single amino acid in the Salmonella effector SarA/SteE triggers supraphysiological activation of STAT3 for anti-inflammatory target gene expressionbioRxiv : the preprint server for biologyFebruary 14, 2024Gaggioli MR, Jones AG, Panagi I, Washington EJ, Loney RE, Muench JH, Brennan RG, Thurston TLM, Ko DC.the vehicle control. Subsequently, the STAT3 and peptide mixtures were added to 1X Glomelt™ (Biotium #33021-1, Fremont, California) a dye that binds hydrophobic residues and fluoresces upon protein denaturation

(5) Protocol for performing and optimizing differential scanning fluorimetry experimentsSTAR ProtocolsDecember 1, 2023Taiasean Wu, Michael Hornsby, Lawrence Zhu, Joshua C. Yu, Kevan M. Shokat, Jason E. GestwickiS6650 Proteostat,480/600,“1×”,Aggregate detection,Enzo,ENZ-51027-K100 GloMelt,468/507,“1×”,Alternative DSF dye,Biotium,Ref. 33021-T "1,8-ANS",350/470,50 μM,Traditional DSF dye,Widely available11,CAS 82-76-8 bis-ANS,355/520,50 μM,Traditional

(6) Structure of the T. brucei kinetoplastid RNA editing substrate-binding complex core component, RESC5PLOS ONEMarch 2, 2023Raul Salinas, Emily Cannistraci, Maria A. Schumachervolume of 20 μL and 1X of GloMelt (GloMeltTM Thermal Shift Protein Stability Kit from Biotium-Cat No. 33021–1). The plates were briefly spun before to transfer to the Bio-Rad CFX Connect Detection System. Fluorescence

(7) Glucocorticoids Bind to SARS-CoV-2 S1 at Multiple Sites Causing Cooperative Inhibition of SARS-CoV-2 S1 Interaction With ACE2Frontiers in ImmunologyJune 15, 2022Hassan Sarker, Rashmi Panigrahi, Eugenio Hardy, J. N. Mark Glover, Shokrollah Elahi, Carlos Fernandez-Patronstability assays were performed: Assay 1: The GloMelt™ Thermal Shift Protein Stability Kit (Catalog#33021-T, Biotium, USA) was used to determine any increase or decrease in thermal stability of SARS-CoV-2 S1

(8) Mechano-growth factor E-domain modulates cardiac contractile function through 14-3-3 protein interactomesFrontiers in physiologyJanuary 1, 2022Solís C, Thompson WC, Peña JR, McDermott-Roe C, Langa P, Warren CM, Chrzanowska M, Wolska BM, Solaro RJ, Pieter Detombe, et al.Each 20 µl reaction volume contained 1 µl of 1:40 Milli-Q-diluted dye (GloMelt Thermal Shift Dye, Kit 33021-1, Biotium), recombinant 14-3-3γ (typically 8 μM, final concentration), or added peptides (from 1 mg/ml

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GloMelt™ Thermal Shift Protein Stability Kit

GloMelt™ Thermal Shift Protein Stability Kit

GloMelt™ Thermal Shift Protein Stability Kit

GloMelt™ Thermal Shift Protein Stability Kit

GloMelt™ Thermal Shift Protein Stability Kit

GloMelt™ Thermal Shift Protein Stability Kit from Biotium, Inc.

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Biotium, Inc.

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GloMelt™ Thermal Shift Protein Stability Kit from Biotium, Inc.

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