Fig 1: CD73/adenosine signaling affected the viability, cell cycle, migration, and induced an epithelial phenotype in HTR-8/SVneo cells. (A) Cell viability was tested using Cell Counting Kit 8 (HY–K0301; MCE, China) at 24 h after treatment with 0, 10, 25, 50, 75, 100, 125 μM adenosine in HTR-8/SVneo Cells. n = 3 biological replicates for each group. (B) The cell cycle of HTR-8/SVneo cells treated with or without 50 μM adenosine were detected using flow cytometry. n = 3 biological replicates for each group. (C) Wound-healing assays for HTR-8/SVneo cells in the presence of 100 μM APCP or 25 μM adenosine. Images were taken at 0 h, 12 h and 24 h after treatment. n = 3 biological replicates for each group. (D) Western blotting of N-cadherin, E-cadherin and Vimentin in the aforementioned groups of cells. n = 3 biological replicates for each group. The original blots were provided in supplementary data as Supplementary Fig. S5B. (E) HTR-8/SVneo cells were treated with or without 50 μM adenosine, 30 nM 5′-N-ethylcarboxamido adenosine (NECA; non-selective adenosine receptor agonist), 100 nM DPCPX (A1-specific antagonist),10 nM ZM 241385 (A2A-specific antagonist), and 10 nM MRS 1706 (A2B-specific antagonist). Western blotting of N-cadherin, E-cadherin and vimentin in the aforementioned groups of cells. n = 3 biological replicates for each group. The original blots were provided in supplementary data as Supplementary Fig. S5C. Results are shown as mean ± SD, One-way ANOVA with post-hoc Tukey HSD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.