Fig 1: Cytokine concentrations in the supernatant of the T lymphocyte cultures in the presence of PMA and ionomycin. (A) IL-10, (B) IFN-γ, (C) TNF-α, (D) IL-17F, and (E) IL-17A concentrations. Wistar CT, (n = 6); Wistar ω-3, (n = 6); GK CT, (n = 6); and GK ω-3, (n = 6). The results are expressed as the means ± standard errors of the means (SEM). CT, control; GK, Goto-Kakizaki; PMA, 12 h with phorbol myristate acetate; IL, interleukin; TNF-α, tumor necrosis factor-α; IFN-γ, interferon-γ.
Fig 2: Evaluation of the expression of genes associated with the Th-1 and Th-17 lymphocyte profile in isolated CD3+ lymphocytes. (A) T-bet, (B) IFN-γ, (C) IL-2, (D) IL-18, (E) TNF-α, (F) ROR-γ, (G) IL-6, (H) TGF-β, and (I) IL-17. Gene expression was analyzed by using real-time PCR and the 2−ΔΔCT method. RPLP0 was used as a constitutive internal control to normalize the data. For test A and I, we used Wistar CT, (n = 15); Wistar ω-3, (n = 11); GK CT, (n = 14); and GK ω-3, (n = 13). For tests C and E, we used Wistar CT, (n = 15); Wistar ω-3, (n = 11); and GK CT, (n = 13). For test D, we used Wistar CT, (n = 15); Wistar ω-3, (n = 9); GK CT, (n = 13); and GK ω-3, (n = 13). For test E, we used Wistar CT, (n = 12); Wistar ω-3, (n = 11); GK CT, (n = 11); and GK ω-3, (n = 15). For tests F and H, we used Wistar CT, (n = 15); Wistar ω-3, (n = 11); GK CT, (n = 12); and GK ω-3, (n = 15). For test G, we used Wistar CT, (n = 14); Wistar ω-3, (n = 10); GK CT, (n = 14); and GK ω-3, (n = 14). The results are expressed as the means ± standard errors of the means (SEM). CT, control; GK, Goto-Kakizaki; IL, interleukin; IFN-γ, interferon-γ; T-bet, T-box transcription factor; ROR-γ, RAR-related orphan receptor-γ; TGF-β, Transforming growth factor β.
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