Description
Human non hematopoietic progenitor cells are depleted by incubating the sample with the optimized biotin conjugated antibody cocktail mixture followed by incubation with streptavidin magnetic nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched human hematopoietic progenitor cells are collected in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems