Description
Mouse non B cells are depleted by incubating sample with the mouse Pan B cell isolation optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched Pan B cells are collected by decanting the liquid in a clean tube. These are cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, and phenotypic characterization. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems