Description
Mouse non dendritic cells are depleted by incubating the sample with the optimized biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoNanoparticles. The magnetically labeled antibody is pulled out by the use of a magnetic separator to leave the target cells intact in the supernatant. The untouched classical and plasmacytoid dendritic cells are collected by decanting the liquid in a clean tube. These are cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. This kit may complement greatly protocols to further isolate classical or plasmacytoid Dendritic Cells by flow cytometry. These magnetic nanoparticles can be used wiht both free standing magnets and column-based systems