Description
DNA methylation at CpG islands in promoters regulates gene expression. It is a dynamic epigenetic process affected by various genetic and environmental factors including aging. The CpG island methylation level at human ELOVL2 gene promoter has been well-associated with chronological age in various populations, cell types, and tissues. ELOVL2 promoter methylation level is therefore a potential marker for human chronological age. ScienCell's Human Age-associated ELOVL2 Promoter Methylation Quantification qPCR Assay Kit (HAEPM) is designed to quantify the level of human ELOVL2 promoter methylation. The reference DNA sample consists of a 1:1 ratio of methylated ELOVL2 promoter copies to non-methylated ELOVL2 promotor copies (both copies represent bisulfite converted promoter sequences), and serves as a reference for calculating the ratio of methylated to non-methylated ELOVL2 promotor of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) negligible non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency