Description
Biological sex is genetically intrinsic to every cell of a sexually reproducing organism, and sex-differentiated characteristics can be found in many complex human phenotypes, including diseases, that influence tissue and cell experiments. The sex of tissues or cells can also be important to note when performing experiments in order to aid in result reproducibility or ensure that findings are not over-generalized. There are occasions, however, when the sex of a sample is unknown and it is crucial to differentiate biological male tissue from biological female tissue. ScienCell's Human GeneQuery™ Biological Sex Determination qPCR Assay Kit (HSDQ) is designed to determine whether a sample of unknown sex is derived from biologically male or female tissue. The Male-specific primer set recognizes only the male, but not the female. The sex chromosome primer set recognizes both male and female genetic material and serves as an indicator of genetic material in the testing samples. The two reference genomic DNA samples provide a reference by providing one biologically male reference and one biologically female reference. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency