Description
The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. This kit is specifically formatted for storage in Invitrogen Supply Centers at -80°C. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression V5 epitope tag for efficient detection of recombinant proteins 6×His sequence for purification using nickel-chelating resin and detection