TUNEL Assay Kit (Fluorescence, 488 nm) 25879S from Cell Signaling Technology

Product Specs
ItemTUNEL Assay Kit (Fluorescence, 488 nm)
CompanyCell Signaling Technology
Price $741.00 Inquire
Catalog Number25879S
Quantity1 Kit
ApplicationsIHC-P, IF-IC, FC-FP

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) GV1001 Reprograms CD47 Immune Checkpoint to Restore Macrophage Antitumor Activity in Oral Squamous Cell CarcinomaInt J Mol SciApril 7, 2026Wei Chen, Seojin Kim, Cheyenne Beheshtian, Angela Jun, Sangjae Kim, No-Hee Parknick-end labeling (TUNEL) using a commercially available assay kit (Cell Signaling Technology, cat. no. 25879), according to the manufacturer’s instructions. 4.9. Reverse Transcription-Quantitative Polymerase Chain Reaction

(2) Anticancer potential of Curcuma caesia: induction of DNA damage and apoptosis in cervical cancerBMC Complementary Medicine and TherapiesMarch 21, 2026Megha Radhakrishnan, Bishnu Prasad Parida, Himanshu Shekhar, Priya Singh, Mumtaz A. Ansari, Jasmeet Singh, Sunita Singh, Gopeshwar Narayanthen harvested, washed with PBS, and proceeded for TUNEL assay using manufacturer’s protocol (CST# 25879). Briefly, cells were fixed with 100 µl of 4% formaldehyde for 20 min at room temperature. Then PBS

(3) SSEA3 and CD105 positivity are associated with the treatment potency of human neural crest-derived nasal turbinate stem cells for Alzheimer's diseaseTransl NeurodegenerMarch 3, 2026Jung Yeon Lim, Jung Eun Lee, Minho Lee, Haewon Shim, Sang In Park, Soon A. Park, Sin-Soo Jeun, Sheng-Min Wang, Sunghwan Kim, Seung Ho Yang, et al.sections were processed using a TUNEL assay kit (PE-conjugated, Cell Signaling Technology, Danvers, MA, 25879). Nuclei were labeled with DAPI (1:1000, Sigma-Aldrich). Fluorescence was detected using a Zeiss LSM510

(4) Identification of cryosensitive niches and a targetable FOS/AP‑1 program in the human ovarian cortex by single‑cell and spatial transcriptomicsBMC MedicineMarch 3, 2026Fanghao Guo, Di Sun, Haixia Ding, Yanquan Li, Baicai Yang, Mengxi Guo, Yongjian Ma, Rui Qiao, Songmao Li, Li Zhang, et al.permeabilized in 0.1% Triton X-100, and processed with the In Situ Cell Death Detection Kit (CST, No. 25879) according to the manufacturer’s instructions. After incubation with the TUNEL reaction mixture at 37

(5) Snail1 Induced Suppression of Proliferation via EGR1, FOXO1, and CEPBγ Creates a Vulnerability for Targeting Apoptotic and Cellular Senescence PathwaysCancers (Basel)February 4, 2026Jack Tran, Samyukta Sundaram, Sukirti Shivpuri, Hunain Khawaja, Cynthia K. Mirantid. Cells were then assayed for apoptosis per the Cell Signaling Technologies (Danvers, MA, USA) TUNEL kit 25879S [41]. Briefly, cells were fixed with 4% formaldehyde and washed with 1× PBS. Cells were then treated

(6) Pharmacologic reversal of advanced Alzheimer’s disease in mice and identification of potential therapeutic nodes in human brainCell Reports MedicineJanuary 20, 2026Kalyani Chaubey, Edwin Vázquez-Rosa, Sunil Jamuna Tripathi, Min-Kyoo Shin, Youngmin Yu, Matasha Dhar, Suwarna Chakraborty, Mai Yamakawa, Xinming Wang, Preethy S. Sridharan, et al.Prolong diamond antifade mountant (Invitrogen, P36961). DNA damage was analyzed using the TUNEL assay kit from Cell Signaling (# 25879), following the exact method described in the kit. Briefly, the free-floating sections were washed three

(7) PARP-1 prevents osteoarthritis pathogenesis by inhibiting apoptosis in chondrocytes: an animal studyArthritis Research & TherapyJanuary 16, 2026Minhye Kim, Mrinmoy Ghosh, Yunji Heo, Myeongyeon Shin, Yunhui Min, Jinu Kim, Young-Ok Sonparaffin-embedded tissue sections and cultured chondrocytes was detected using a TUNEL assay kit (#25879, Cell Signaling Technology) according to the manufacturer’s instructions. For in vitro analysis, chondrocytes

(8) Development of a Synthetic Lethality‐Based Combination Therapy Using LIG1 and PARP Inhibitors for Prostate CancerCancer ScienceDecember 1, 2025Masaru Tani, Koji Hatano, Yu Ishizuya, Toshiki Oka, Tomohiro Kanaki, Shunsuke Inoguchi, Akihiro Yoshimura, Yuki Horibe, Yutong Liu, Sassi Nesrine, et al.TUNEL Assay of FFPE Samples TUNEL assay was performed on FFPE tissue sections using the TUNEL assay Kit (#25879; Cell Signaling Technology [CST], Beverly, MA, USA), according to the manufacturer's instructions. Tissue

(9) MIF/CD74 axis regulates alveolar epithelial type II cell apoptosis, autophagy, and transdifferentiation in bronchopulmonary dysplasia via the TGFβ1/SMAD4 pathwayRespiratory ResearchNovember 25, 2025Mengyao Fan, Shaohua Wang, Zhenzhuang Zou, Jian DengSubsequently, the cells were incubated with the TUNEL reaction mixture (TUNEL Assay Kit, fluorescence, 488 nm; #25879, Cell Signaling Technology) according to the manufacturer’s instructions. The cells were then washed

(10) GV1001, an hTERT-Derived Peptide, Prevents Cisplatin-Induced Nephrotoxicity by Preserving Mitochondrial FunctionCellsNovember 19, 2025Wei Chen, Cheyenne Beheshtian, Seojin Kim, Reuben Kim, Sangjae Kim, No-Hee Parka Terminal Deoxynucleotidyl Transferase dUTP Nick-End Labeling (TUNEL) assay kit (Cell Signaling, #25879, Danvers, MA, USA) according to the manufacturer’s protocols. 2.6. Quantitative Real-Time Polymerase Chain Reaction

(11) Persistence of Cardiac Malprogramming in Late Gestational Fetal Sheep by Early to Mid- Gestational Testosterone ExcessAmerican Journal of Physiology-Heart and Circulatory PhysiologyOctober 1, 2025Bashar Alkhatib, Divya Purswani, Alekya Dantam, Md Rahatullah Razan, Mary Jabari, Vasantha Padmanabhan, Arpita Kalla Vyasstained using the commercially available TUNEL staining kit (Cell Signaling Technology, Catalog No. 25879) following the manufacturer’s instructions. SignalStain Citrate Unmasking Solution (10×, Cell Signaling,

(12) Targeted inhibition of hepatic de novo ceramide synthesis ameliorates MASHScience AdvancesSeptember 26, 2025Xiaodong Yu, Chenyuan Huang, Martijn Evers, Jingjing Liu, Hui Jun Ting, Sitong Zhang, Suet Yen Chong, Michelle Siying Tan, Siyu Wang, Nilofer Sayed, et al.staining. Apoptotic cells were detected using the TUNEL assay kit (Cell Signaling Technology, catalog no. 25879), according to the manufacturer’s protocol. Apoptotic cells were fluorescently labeled in green and

(13) Inhibition of XPOT promotes breast cancer cell pyroptosis to suppress cancer progressionCentral European Journal of ImmunologySeptember 24, 2025Meng Liang, Min Wang, Beibei Zhudetected at 450 nm. TUNEL assay Cell death was assessed using the TUNEL fluorescence assay kit (CST, 25879S, USA). Cells were transfected or treated with azalamellarin N, washed with PBS, fixed, and permeabilized.

(14) A lupus-derived autoantibody that binds to intracellular RNA activates cGAS-mediated tumor immunity and can deliver RNA into cellsScience SignalingMarch 25, 2025Xiaoyong Chen, Xiangjun Tang, Ying Xie, Benedette J. Cuffari, Caroline Tang, Fei Cao, Xingchun Gao, Zhouqi Meng, Philip W. Noble, Melissa R. Young, et al.DAB and methylgreen counterstain. TUNEL stain was performed using Cell Signaling TUNEL Assay Kit (#25879) and signal quantified by ImageJ. Studies were conducted under a Yale University IACUC approved protocol. PRR

(15) Small spheroids for head and neck cartilage tissue engineeringScientific ReportsDecember 30, 2024Sven Reutter, Johann Kern, Yvonne Jakob, Nicole Rotter, David Gvaramia(TUNEL) assay. TUNEL procedure was performed on fixed spheroid samples using the TUNEL Assay Fluorescence Kit (#25879, 488 nm, Cell Signaling Technology, USA) according to the manufacturer’s instructions. Total cell nuclei

(16) Hybrid Nanoparticle Engineered with Transforming Growth Factor -β1-Overexpressed Extracellular Vesicle and Cartilage-Targeted Anti-Inflammatory Liposome for OsteoarthritisACS NanoDecember 17, 2024Jun Yong Kim, Won-Kyu Rhim, Seung Yeon Lee, Jung Min Park, Duck Hyun Song, Seung-Gyu Cha, Sang-Hyuk Lee, Dong-Youn Hwang, Byoung Ju Kim, Seungsoo Rho, et al.detected according to the protocol provided by the manufacturer. PRCs subjected to the TUNEL assay (25879, Cell Signaling Technologies, MA, USA) were imaged using a fluorescence microscope (CKX53, OLYMPUS,

(17) Mitochondrial transfer balances cell redox, energy and metabolic homeostasis in the osteoarthritic chondrocyte preserving cartilage integrityTheranosticsOctober 7, 2024Angela C. Court, Ana María Vega-Letter, Eliseo Parra-Crisóstomo, Francesca Velarde, Cynthia García, Alexander Ortoloff, Rolando Vernal, Carolina Pradenas, Patricia Luz-Crawford, Maroun Khoury, et al.mice knee joints samples was detected using the TUNEL assay kit (Fluorescence, 488nm; Cell Signaling #25879), according to manufacturer's instruction. Paraffin-embedded histological sections were deparaffinized,

(18) Circ_0008315 promotes tumorigenesis and cisplatin resistance and acts as a nanotherapeutic target in gastric cancerJournal of NanobiotechnologyAugust 29, 2024Yao Fei, Danping Cao, Yanna Li, Zhixiong Wang, Runyu Dong, Menglin Zhu, Peng Gao, Xiaoming Wang, Juan Cai, Xueliang ZuoUSA), N-cadherin (#5741, Cell Signaling Technology), Ki-67 (#34330, Cell Signaling Technology), TUNEL (#25879, Cell Signaling Technology) primary antibodies overnight at 4℃. After that, the cells were incubated

(19) SARS-CoV-2 propagation to the TPH2-positive neurons in the ventral tegmental area induces cell death via GSK3β-dependent accumulation of phosphorylated tauPloS oneJanuary 1, 2024Imai M, Kawakami F, Uematsu T, Matsumoto T, Kawashima R, Kurosaki Y, Tamaki S, Maehana S, Ichikawa T, Hanaki H, et al.mounting medium (Dako, CA, USA). TUNEL staining TUNEL staining was performed using the TUNEL assay kit (#25879, CST) according to the manufacturer’s instruction, followed by staining with DAPI (D532, Dojindo). Finally,

(20) Transmembrane BAX inhibitor motif containing 6 suppresses presenilin-2 to preserve mitochondrial integrity after myocardial ischemia-reperfusion injuryInternational Journal of Biological SciencesFebruary 13, 2023Li Ma, Lihan Liao, Na Zhou, Huikang Tao, Hao Zhou, Ying Tan, Weidan Chen, Fan Cao, Xinxin Chenanalyzed using an ELISA Kit (#ab239704, Abcam) 35. Cell death rate was detected using a TUNEL assay (#25879, CST) 6. Western blots Proteins were extracted according to the conventional method 44. In brief,

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TUNEL Assay Kit (Fluorescence, 488 nm) from Cell Signaling Technology

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TUNEL Assay Kit (Fluorescence, 488 nm) from Cell Signaling Technology

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