Fig 1: Bcl-xL overexpression alters T cell populations in Lckpr-bcl-xL mice across aging.(A) Total splenic cell counts and (B) T cell count obtained from the spleens of C57BL6/J and Lckpr-bcl-xL mice of all age groups (n = 15 to 20). (C) CD3+hi and CD3+lo levels of splenic T cells from C57BL6/J and Lckpr-bcl-xL mice of all age groups (n = 15). mo, months. (D) Distribution of T helper and cytotoxic T cells, (E) distribution of naïve/memory/effector within T helper cells, and (F) distribution of naïve/memory/effector within cytotoxic T cells in C57BL6/J and Lckpr-bcl-xL mice of all age groups (n = 8 to 10). Data are presented as the means ± SD.
Fig 2: Bcl-xL overexpression modulates Treg distribution and promotes Treg infiltration in skeletal muscle to reduce IFN-γ inflammation.Percentage of FoxP3+ Treg cells in (A) whole blood and in (B) spleens from C57BL6/J and Lckpr-bcl-xL mice of all age groups (n = 4 to 5). (C) Representative images of CD3+, FoxP3+, and DAPI staining in muscle samples from C57BL6/J and Lckpr-bcl-xL mice of all age groups. Quantification of (D) infiltrated CD3+ cells and (E) FoxP3+ cells and (F) ratio of FoxP3+/CD3+ (n = 3 fields with n > 50 cells). (G) IFN-γ levels measured in plasma from C57BL6/J and Lckpr-bcl-xL mice (n = 6). Data are presented as the means ± SD.
from Cell Signaling Technology for Mouse Naive/Effector/Memory T Cell Markers Flow Cytometry Panel