Fig 1: The impact of IRI surgery on renal physiological function and the expression levels of renal fibrosis-related factors in kidney tissue(A and B) The effect of IRI surgery on renal function, where A shows the creatinine levels in the blood of mice in the Sham, IRI_2d, and IRI_1w groups; B shows the blood urea nitrogen levels in the blood of mice in the Sham, IRI_2d, and IRI_1w groups.(C–H) Expression of Spp1, TGF-β, Smad2/3, P-Smad2/3, Col1a1, and α-SMA in kidney tissues using ELISA.(I and J) Expression of Spp1, TGF-β, Smad2/3, P-Smad2/3, Col1a1, and α-SMA in kidney tissues using immunohistochemistry. In all the images, compared to the Sham group, ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001; the bar in the image equals 750 μm.
Fig 2: Investigation into the impact of Spp1 on fibroblast behavior(A and B) Scratch experiment to evaluate the effect of Spp1 on fibroblast migration.(C and D) Transwell chamber experiment to assess the effect of Spp1 on fibroblast migration.(E) CCK8 experiment to measure the effect of Spp1 on fibroblast proliferation.(F) qRT-PCR to detect the expression levels of Col1a1 and α-SMA in fibroblasts. In all the images, compared to the sh-NC or oe-NC groups, ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001; bar in the image equals 750 μm or 500 μm; each experiment was conducted three times with six replicates each time.
from Cell Signaling Technology for FastScan ™ COL1A1 ELISA Kit