Description
This assay is based on the competitive enzyme immunoassay for the detection of Am in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Am in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Am antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Am in it. This value is compared to the standard curve and the Am concentration is subsequently obtained