Description
Principle of the Assay: The Alanine Aminotransferase (ALT) Assay Kit measures ALT activity through a series of enzyme driven reactions. ALT in samples reacts with alanine to transfer an amino group to another substrate, producing glutamate and pyruvate. Pyruvate is then detected with the fluorometric probe. Samples and standards are incubated for 30-60 minutes and then read with a standard 96-well fluorometric plate reader (Ex. 530-570 nm/Em. 590-600 nm). The ALT activity is directly proportional to the amount of pyruvate generated in the reaction. Sample ALT levels are determined by comparison with the known pyruvate standards