Description
Principle of the Assay: The Lysine Assay Kit measures lysine within food or biological samples. Lysine is converted by lysine oxidase into 6-amino-2-oxohexanoate plus ammonia and hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples and standards are read with a standard 96-well fluorometric plate reader. Samples are compared to a known concentration of lysine standard within the 96-well microtiter plate format (Figure 1)