Description
Principle of the Assay: Cell Biolabs' D-Amino Acid Assay Kit (Colorimetric) measures free D-Amino Acids (except for D-Glutamate and D-Aspartate) present in foods or biological samples in a 96-well microtiter plate format. D-amino acids are oxidatively deaminated by D-Amino Acid oxidase into the corresponding alpha-keto acid, ammonia, and hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific colorimetric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples and standards are read with a standard 96-well colorimetric plate reader. Samples are compared to a known concentration of D-Alanine standard within the 96-well microtiter plate format (Figure 1)