Description
Intended Uses: Used for the quantitative determination of Spike (Trimer) specific IgM antibody concentration in serum and plasma.
Principle of the Assay: This kit employs the quantitative indirect enzyme immunoassay technique. A recombinant Spike (Trimer) protein has been pre-coated onto a microplate. Positive Control and samples are pipetted into the wells and the Spike (Trimer) specific antibody will be captured by immobilized Spike (Trimer) protein. After washing away any unbound substances, a biotin-conjugated anti-human IgM antibody is added to the wells. Next, adding HRP labeled Streptavidin(SA) into the well and then removing any unbound antibody-enzyme reagent by washing, a Color Reagent TMB is added to the wells and color develops in proportion to the concentration of Spike (Trimer) specific IgM antibody in the sample