Description
Principle of the Assay: Polyphenol Oxidase (PPO, EC1.10.3.1) is widely found in animals, plants, microorganisms and cultured cells. As a copper-containing oxidase, PPO can cause browning by oxidizing Monohydric Phenols and Dihydric Phenols to produce Quinones. This enzyme is also closely related to fruit and vegetable processing, tea quality and tissue culture. CheKine™ Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit provides a simple method for detecting PPO activity in a variety of biological samples such as Serum, Plasma, Juices, Animal and Plant Tissues, Cells and Bacteria. In the assay, Polyphenol Oxidase (PPO) catalyzes catechol to generate quinone which has a characteristic absorption peak at 525 nm.; The rate of quinone increase at 525 nm can reflect PPO activity