Description
Intended Uses: Insulin ELISA Kit is intended for the quantitative measurement of Insulin in human serum or plasma.
Principle of the Assay: Insulin ELISA is based on solid phase sandwich ELISA method. The samples and conjugate reagent (anti-Insulin biotin & HRP) are added to the wells coated with Streptavidin. Insulin in the patient's serum binds to the matched pair Abs, forming a sandwich complex and simultaneously the complex is being immobilized on the plate through streptavidin-biotin interactions. Unbound protein and HRP conjugate are washed off, through a washing step. Upon addition of the substrate, the intensity of color is proportional to the concentration of Insulin in the samples. A standard curve is prepared by relating the color intensity to the concentration of Insulin