Description
Principle of the Assay: TrxR can catalyzes the reduction of DTNB by NADPH to generate TNB and NADP+. TNB has a characteristic absorption peak at 412 nm. However, the Reduced Glutathione can also react with DTNB to form TNB, so this kit uses 2-vinylpyridine to inhibit the reduced Glutathione present in samples. TrxR activity can be calculated by measuring the increase rate of TNB at 412 nm