Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Anandamide (also known as AEA) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse AEA antibody. AEA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse AEA Antibody is added and binds to AEA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AEA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse AEA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm