Description
Principle of the Assay: The Guanosine Assay Kit measures total Guanosine within biological samples. Guanosine is converted to Guanine by PNP. Guanine is converted into xanthine by guanine deaminase. Then xanthine is converted to uric acid and hydrogen peroxide by xanthine oxidase. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of Guanosine standard within the 96-well microtiter plate format. Samples and standards are incubated for 15 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1)