Description
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CD44 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD44 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for CD44 is added to the wells and binds to the combination of capture antibody CD44 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of CD44 present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven CD44 standard dilutions and CD44 sample concentration determined