Description
Intended Uses: This kit can be used to measure the concentration of ferrous iron in serum, plasma and tissue samples.
Principle of the Assay: Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly