Description
This assay employs a two-site sandwich ELISA to quantitate CDSN in samples. An antibody specific for CDSN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDSN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDSN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDSN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Corneodesmosin is a protein found in corneodesmosomes, which localize to human epidermis and other cornified squamous epithelia. During maturation of the cornified layers, the protein undergoes a series of cleavages, which are thought to be required for desquamation. The gene is located in the major histocompatibility complex (MHC) class I region on chromosome 6.Corneodesmosomes are intercellular structures that are involved in desquamation, the shedding of superficial corneocytes from the skin surface. Corneocytes are anucleated cells derived from keratinocytes during the late stages of terminal differentiation of cornified squamous epithelia such as the epidermis