Description
Test Principle: This assay employs a two-site sandwich ELISA to quantitate FDP in samples. An antibody specific for FDP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FDP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FDP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FDP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Fibrin degradation product (FDPs), also known as fibrin split products, are components of the blood produced by clot degeneration. These are produced by the action of plasmin on deposited fibrin.The levels of these FDPs rises after any thrombotic event.It can be used to test for disseminated intravascular coagulation