Description
Principle of the Assay: Mitochondrial membrane potential is an important parameter of mitochondrial function used as an indicator of cell health. EliteTM Homogeneous Mitochondrial Membrane Potential Assay Kit uses a fluorescent cationic, lipophilic dye (m-MPI) to signal the loss of mitochondrial potential. This kit is the non-wash version. In normal or healthy cells, m-MPI concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. However, in apoptotic and necrotic cells, m-MPI diffuses out of mitochondria and changes to green fluorescent monomeric form. This kit provides all the necessary reagents for the analysis of mitochondrial integrity in cells. Fluorescence excitation/emission maxima: 514/529 nm for monomer form; 585/590 nm for J-aggregate form