Description
Principle of the Assay: Alanine Aminotransferase (ALT/GPT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum ALT activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, ALT has the highest content in cardiomyocytes, and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice. CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as Animal Tissues, Plant Tissues, Cell culture (Adherent or Suspension Cells), Serum, Bacteria. In the assay, ALT catalyzes the transamination reaction of alpha-ketoglutarate and alanine at 37 degree C and pH7.4 to produce Glutamic Acid and Pyruvate; Pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate Phenylhydrazone, which appears brownish red under Alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of Pyruvate Phenylhydrazone increase at 505 nm can reflect ALT/GPT activity