Description
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BAD in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to BAD. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to BAD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BAD in the samples is then determined by comparing the O.D. of the samples to the standard curve