Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Bone specific alkaline phosphatase (also known as BAP) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human BAP antibody. BAP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human BAP Antibody is added and binds to BAP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BAP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human BAP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm