NEBNext Ultra II FS DNA PCR-Free Library Prep Kit from New England Biolabs

Product Specs
ItemNEBNext Ultra II FS DNA PCR-Free Library Prep Kit
CompanyNew England Biolabs
Price $609.00 Inquire
Catalog NumberE7430S
Temperature-20 C
ApplicationsDNA Analysis, PCR, NGS
Quantity24 reactions

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Pet Exposure Is Associated with Altered Gut Microbiota and Higher Phospholipid and Protein Concentrations in the Breast Milk of Overweight/Obese Pregnant WomenMetabolitesMay 9, 2026Yanpin Liu, Di Yang, Junying Zhao, Yan Liu, Yaling Wang, Yan Liu, Qian Liu, Xiaofei Fan, Bin Liu, Minghui Zhang, et al.NEBNext Ultra II FS DNA PCR-Free Library Prep Kit (New England Biolabs, Ipswich, MA, USA; catalog no. E7430L) according to manufacturer protocol. Libraries were quantified by Qubit fluorometry and quantitative PCR

(2) Qiling Hushen Formula Ameliorates Type 2 Diabetic Kidney Disease via Gut-Kidney Axis Restoration and TLR4/NF-κB/NLRP3 SuppressionJ Diabetes ResMay 3, 2026Yuxin Wang, Pei Sheng, Xue Wang, Min Lin, Wentao Cai, Shiyao Wei, Yuyun Du, Ming Yan, Lei Wang, Xiaofei Anfollowed by library construction using the NEB Next Ultra II FS DNA PCR‐free Library Prep Kit (Cat#: E7430L). The constructed library was quantified using qubit and real‐time PCR. The quantified libraries were

(3) The lipid transfer protein STARD7 controls intestinal tumor development in a context-dependent mannerEMBO Molecular MedicineMarch 30, 2026Kateryna Shostak, Yu Chen, Chloé Maurizy, Gilles Rademaker, Xinyi Xu, Arnaud Blomme, Pierre Close, Olivier Renson, Matthias Van Hul, Patrice D Cani, et al.generated using NEB Next® Ultra™ II FS DNA PCR- free Library Prep Kit (New England Biolabs, USA, Catalog #: E7430L) following manufacturer’s recommendations and indexes were added. The library was checked with Qubit

(4) Dynamic gut responses to sepsis uncovered by multi-omics profiling in a rodent modelCommunications BiologyMarch 27, 2026Jingchao Lei, Jing Qi, Jia Zhai, Ziying Chen, Nanyi Li, Yao Zhang, Yishu Yuan, Chuanzheng Sunwere generated using NEB Next® Ultra™ II FS DNA PCR- free Library Prep Kit (New England Biolabs, USA, E7430L) following manufacturer’s recommendations and indexes were added. The library was checked with Qubit

(5) Slope Aspect Shapes Soil Bacterial Diversity and Functional Profiles on the Pamir PlateauiScienceMarch 20, 2026Jing Zhu, Huinan Wang, Qiong Jia, Yanpeng Ding, Xiaojing Liu, Qiyong Tang, Meiying Gu, Zhidong ZhangBiolabs,Cat# M0531L NEB Next® Ultra™ II FS DNA PCR-free Library Prep Kit,New England Biolabs,Cat# E7430L Universal DNA Purification Kit,"Tiangen Biochemical, Beijing, China",Cat# DP214 TIANamp Bacteria DNA

(6) Dietary energy levels regulate feed intake of broilers through the brain-gut axisFront Vet SciMarch 5, 2026Yiwen Yang, Ling Zhou, Hongjing Liu, Zhiyong Huang, Jiancong Zhang, Li Lvfor sequencing library construction with the NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit (NEB/E7430L). Sequencing was performed on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) after

(7) Gut microbial signatures of advanced hepatocellular carcinoma and their potential diagnostic valueFront MicrobiolFebruary 2, 2026Yan Wang, Zhen Yang, Chuang Liu, Yufeng Liu, Zhongyuan Bai, Wentao Miao, Tiantian Zhang, Yan Wang, Xiang Li, Zhiyong Lai, et al.NEBNext® Ultra™ II FS DNA PCR-Free Library Prep Kit (New England Biolabs, United States; Catalog No. E7430L). Libraries were quantified via Qubit 2.0 fluorometry and qPCR prior to sequencing on the NovaSeq 6000

(8) Distinct patterns of microbial association across deep-sea corals from the Western Pacific Magellan SeamountsMicrobiol SpectrDecember 4, 2025Weizhi Song, Shan Zhang, Maeva Perez, Jiasui Li, Haiying Ma, Torsten Thomas, Jian-Wen Qiu, Pei-Yuan Qianprepared using the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, USA, Catalog E7430L) following the manufacturer’s recommendations and sequenced on Illumina NovaSeq X Plus and NovaSeq 6000

(9) Reovirus infection results in rice rhizosphere microbial community reassembly through metabolite-mediated recruitment and exclusionMicrobiomeOctober 23, 2025Zhanbiao Li, Wandi Luo, Huiting Xie, Cuiping Mo, Bixia Qin, Yige Zhao, Xiao Chen, Songbai Zhang, Yaling Zhao, Mengcen Wang, et al.generated using the NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit (New England Biolabs, USA, E7430L) according to the manufacturer’s instructions, with indices added for sample identification. Library

(10) Impact of mixed silages of licorice aerial parts and whole plant corn on nutrient digestibility, rumen fermentation, and gastrointestinal microbiota in Simmental cattleFront Vet SciOctober 9, 2025Haonan Liu, Limin Tang, Xingyu Xu, Qifeng Gao, Yuliang Sun, Wenhao Li, Dong Lu, Houjun Yu, Tao JiangNEB Next® Ultra™ II FS DNA PCR-free Library Prep Kit (New England Biolabs, United States, Catalog #: E7430L) following manufacturer’s recommendations, and unique indexes were added to each sample. The libraries

(11) Exploring the skin mycobiome in intensive care patients: a pilot study on fungal diversity from axillary and groin swabsBMC MicrobiologySeptember 29, 2025Teresa Nascimento, João Inácio, Daniela Guerreiro, Patrícia Patrício, Luís Proença, Cristina Toscano, Helena Barrosousing NEB Next® UltraTM II FS DNA PCR- free Library Prep Kit (New England Biolabs, USA, Catalog #: E7430L) following manufacturer’s recommendations and indexes were added. The library was checked with Qubit

(12) Bacteria dynamics and its correlation with chemical composition changes in tobacco leaves during flue curingApplied Microbiology and BiotechnologySeptember 24, 2025Yunfei Sha, Mengqian Zhou, Demin Liang, Jie Yu, Yanjiu Bi, Qiansi Chen, Huina Zhou, Xianchao Shang, Da Wuusing the NEB Next® Ultra™ II FS DNA PCR-Free Library Prep Kit (New England Biolabs, USA, Catalog #: E7430L), and library sequencing was conducted on the NovaSeq 6000 platform. Bacterial community analysis and

(13) Optimization of DNA Fragmentation Techniques to Maximize Coverage Uniformity of Clinically Relevant Genes Using Whole Genome SequencingDiagnostics (Basel)September 10, 2025Vanessa Process, Madana M.R. Ambavaram, Sameer Vasantgadkar, Sushant Khanal, Martina Werner, Maura A. Berkeley, Zachary T. Herbert, Greg Endress, Ulrich Thomann, Eugenio DavisoIllumina, San Diego, CA, USA), enzymatic-based NEBNext Ultra II FS DNA PCR-free Library Prep Kit (Cat. No. E7430L, New England Biolabs, Ipswich, MA, USA), and enzymatic-based Watchmaker DNA Library Prep Kit with Fragmentation,

(14) Study on the alteration of gut microbiota in ovariectomized rats and the impact of estrogen intervention over a 3-month periodPLOS OneSeptember 4, 2025XinDong Lei, TingTing Cheng, Han Dong, JieYing Xia, Yang Hong, GuoQiang Cheng, YongJin Wang, TieZhu Chenelectrophoresis on a 2% agarose gel. The NEB Next® Ultra™ II FS DNA PCR-free Library Prep Kit (NEB/E7430L) was used for library construction. Use FLASH after truncating the Barcode and primer sequences [16],

(15) Determining the Effectiveness of Saccharomyces cerevisiae as a Postbiotic in Mass-Reared Acheta domesticus (House Cricket)InsectsJuly 9, 2025Kimberly L. Boykin, Erik Neff, Mark A. Mitchellgenerated using NEB Next® Ultra™ II FS DNA PCR-free Library Prep Kit (New England Biolabs, USA, Catalog #: E7430L) following the manufacturer’s recommendations, and indices were added. The library was checked with

(16) Intermittent supplementation with Akkermansia muciniphila and galactooligosaccharides modulates Alzheimer’s disease progression, gut microbiota, and colon short-chain fatty acid profiles in miceFrontiers in Aging NeuroscienceJune 30, 2025Arnas Kunevičius, Akshay Kumar Vijaya, Alessandro Atzeni, Jonas Mingaila, Ieva Šimoliūnė, Rapolas Jamontas, Emilija Keževičiūtė, Miguel Gueimonde, Rolandas Meškys, Daiva Baltriukienė, et al.NEB Next® Ultra™ II FS DNA PCR-free Library Prep Kit (New England Biolabs, United States, Catalog #: E7430L) following manufacturer’s recommendations and indexes were added. The library was checked with Qubit

(17) Lower respiratory tract co-infection of Streptococcus pneumoniae and respiratory syncytial virus shapes microbial landscape and clinical outcomes in childrenFront Cell Infect MicrobiolJune 9, 2025Xiaoran Yu, Xuemei Yang, Yiqin Song, Jie Yu, Tingting Jiang, He Tang, Xiaoxuan Yang, Xi Zeng, Jing Bi, Adong Shen, et al.generated using NEB Next® Ultra™ II FS DNA PCR-free Library Prep Kit (New England Biolabs, USA, Catalog #: E7430L) following manufacturer’s recommendations and indexes were added, then the final libraries were sequenced

(18) Exploring the role of the vaginal microbiome in HPV infection dynamics: A prospective cohort studyiScienceMay 16, 2025Yiran Liu, Ran Li, Jun Liu, Xiaolong Liang, Mingrui Su, Shuzhen Wang, Boran Muwere generated utilizing the Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, Cat#E7430L USA). Following precise quantification via Qubit and Q-PCR, a NovaSeq 6000 Sequencer (Illumina Inc.

(19) Alcohol-induced bone loss driven by dysregulated spatial distribution of gut microbiota and PGD2-IL17 pathway-mediated osteoclast activationFront MicrobiolMay 15, 2025Haoyu Guo, Yujing Bi, Gongzi Zhang, Shicheng Luo, Xiaopeng Jia, Ruifu Yang, Ye Peng, Lihai Zhangperformed using the NEB Next® Ultra™ II FS DNA PCR-free Library Prep Kit (New England Biolabs [NEB]; E7430S). The constructed library was quantified by Qubit and Q-PCR. After the library passed the quality check,

(20) The intratumoral microbiota heterogenicity is related to the prognosis and tumorigenesis of cervical cancerFront Cell Infect MicrobiolMay 13, 2025Yi Guo, Yuhang Xiao, Changyi Zhang, Ying Wang, Guangxu Cao, Ka Yu Tse, Zhiqiang Han, Fang Li, Yong Zhiusing NEB Next® UltraTM II FS DNA PCR- free Library Prep Kit (New England Biolabs, USA, Catalog #: E7430L) following manufacturer’s recommendations and indexes were added. The library was checked with Qubit

Be the First to Write a Review!