Human placenta with OriGene Mouse anti-PD-L1 (UM800120 dilution 1:100) using Polink1 (D12-110) mouse polymer detection and HIER TEE buffer pH9 for was used 10 minutes pressure cooker. Primary antibody was incubated 1 hour; Polink-1 (D12-110) for 15 minutes and chromogen for 5 minutes according to protocol data sheet.
Supplier Page from OriGene Technologies for Polink-1 HRP Mouse DAB Detection Kit