Human tonsil with rabbit anti FoxP1 (clone EP137) using Polink1 (D13-18) rabbit polymer detection and HIER Accel (B22C-125)for was used 10 minutes pressure cooker. Primary antibody was incubated 1 hour; Polink-1 (D13-18) for 15 minutes and chromogen for 5 minutes according to protocol data sheet.
Supplier Page from OriGene Technologies for Polink-1 HRP Rabbit DAB Detection Kit