Human placenta screened with OriGene's Ms anti PDL1 (UM800120 @ 1:100). HIER buffer TEE (B21-100) was used in pressure cooker. Secondary Polink 1 AP Mouse with out chromogen (D18-110) was use according to protocol data sheet. Cytoplasmic and membrane stain was visualized with GBI permanent red chromogen (C13-120).
Human colon cancer screened with OriGene's Ms anti CEA (TA803456 @ 1:400). HIER buffer TEE (B21-100) was used in pressure cooker. Secondary Polink 1 AP Mouse with out chromogen (D18-110) was use according to protocol data sheet. Cytoplasmic and membrane stain was visualized with GBI permanent red chromogen (C13-120).
Human tonsil screened with OriGene's Ms anti MS4A1 (CD20) (UM800009 @ 1:800). HIER buffer TEE (B21-100) was used in pressure cooker. Secondary Polink 1 AP Mouse with out chromogen (D18-110) was use according to protocol data sheet. Cytoplasmic and membrane stain was visualized with GBI permanent red chromogen (C13-120).
Supplier Page from OriGene Technologies for Polink-1 AP Mouse Permanent Red Detection Kit