Human tonsil screened with OriGene Ms anti Ki67 (UM800033 @ 1:1000). HIER buffer Citrate (B05-100B) was used 10 minutes pressure cooker. Secondary Polink 2 plus AP Ms (D69-18) was use according to protocol data sheet. Nuclear stain was visualized with GBI permanent red chromogen (C13-120)
Human colon cancer screened with OriGene Ms anti MS4A1 (UM800009@ 1:800). HIER buffer Citrate (B05-100B) was used 10 minutes pressure cooker. Secondary Polink 2 plus AP Ms (D69-18) was use according to protocol data sheet. Cytoplasmic and membrane stain was visualized with GBI permanent red chromogen (C13-120).
Supplier Page from OriGene Technologies for Polink-2 Plus AP Mouse Permanent Red Detection Kit