Fig 1: Protein expression profiles of TNFα, BDNF, HO-1, Nrf2, IL-6 and MCP-1 in rat brain tissue samples. Values were represented as Mean ± SD and significance was calculated using One-way ANOVA with Tukey’s multiple comparisons where **p < 0.01, ***p < 0.001 and ****p < 0.0001 (n = 6).
Fig 2: GarA shows neuro-protective effect in rats against alcohol induced damage. Gene expression over β-actin in vivo in different treatment groups showing (A). Vimentin (B). TNFα (C). MCP-1 (D). HO-1 (E). Nrf2 (F). BDNF (G). CLDN5 (H). IL-10 (I). PCSK9. Values are represented as Mean ± SD and significance was calculated using One-way ANOVA with Tukey’s multiple comparisons where nsp > 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 (n = 6).
Fig 3: GarA helps reduce inflammation and has an antioxidant and neuroprotective activity in SH-SY5Y cells against ethanol. Gene expression over 18S in vitro in different treatment groups showing (A). TNF-α (B). MCP-1 (C). Nrf2 (D). HO-1 (E). BDNF (F) CLDN. Values are represented as Mean ± SD and significance was calculated using One-way ANOVA with Dunnet’s multiple comparisons where nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 (n = 3).
Fig 4: Schematic diagram showing cellular and molecular mechanisms occurring in the brain and BBB in the state of intoxication (upper half) and GarA provided as a drug along with ethanol (lower half), separated by dashed line. Ethanol exposure is associated with glial activation, increased pro-inflammatory cytokines such as TNFα, IL-6 and potential disruption of BBB. GarA is shown to be linked with modulation of neuronal and glial responses, including factors such as BDNF, Nrf2, HO-1 and IL-10. Image drawn using SketchBook app v6.0.4.
Supplier Page from Biorbyt for Rat NFE2L2 ELISA Kit
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NFE2L2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NFE2L2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NFE2L2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NFE2L2 in the samples is then determined by comparing the OD of the samples to the standard curve