Fig 1: Comparison of plasma protein concentrations between mining region residents (MRR) and non-mining region residents (NMR). Box plots show that plasma levels of NFKB1 (A) and CDKN2A (B) were significantly higher in the MRR group compared to the NMR group. No significant group differences were observed for IGF2 (C)and APOA5 (D).
from Biorbyt for Human APOA5 ELISA Kit
Application Notes: standard: 80 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOA5 in the samples is then determined by comparing the OD of the samples to the standard curve