Shiga toxins ELISA Kit DEIASL162 from Creative Diagnostics

Product Specs
ItemShiga toxins ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIASL162
PrecisionIntra-Assay
For the determination of intra-assay performance, 6 positive fecal specimens and 6 negative fecal specimens were analyzed. Each specimen was assayed in replicates of eight. All positives remained positive and all negatives remained negative.
Inter-Assay
The inter-assay precision of the Shiga toxins ELISA Kit was determined using 12 fecal specimens (six negative, two positive for Stx1, two positive for Stx2, and two positive for both Stx1 and Stx2). The samples were tested, twice a day over a 5-day period using 2 different kit lots. A positive and negative control was run on each day. All positives remained positive and all negatives remained negative
ValidationThe reproducibility of the SHIGA TOXIN CHEK test was determined using 11 fecal specimens that were coded to prevent their identification during testing. Testing was performed at 2 independent laboratories and on-site at TECHLAB^®, Inc. The samples were tested, twice a day over a 5-day period by multiple technicians at each site using 2 different kit lots. A positive and negative control was run with each panel of the masked samples. The results from each laboratory were submitted to TECHLAB^®, Inc. and compared with in-house results. The results were consistent among the different locations, and exhibited a correlation of 100%. The samples produced the expected results 100% of the time
ComponentsMicroassay Plate –12 strips, each strip consisting of 8 wells, coated with monoclonal antibodies specific for Stx1 and Stx2 (stored with desiccant).
Diluent (40 mL) – buffered protein solution containing 0.02% thimerosal.
Substrate (14 mL) – solution containing tetramethylbenzidine and peroxide.
Wash Buffer Concentrate (50 mL) – 20X concentrate containing phosphate buffered saline, detergent, and 0.2% thimerosal.
Stop Solution (7 mL) – 0.6N sulfuric acid. CAUTION: Avoid contact with skin; flush with water immediately if contact occurs.
Positive Control (3.5 mL) – inactivated antigen in a buffered protein solution containing amphotericin B.
Conjugate (7 mL) – polyclonal antibodies specific for Stx1 and Stx2 coupled to horseradish peroxidase in a buffered protein solution containing 0.02% thimerosal
SensitivityThe cutoff for the test for direct fecal specimens was established at concentrations of 0.28 ng/mL Stx1 and 0.23 ng/mL Stx2, and for broth cultures at concentrations of 0.18 ng/mL Stx1 and 0.30 ng/mL Stx2
Quantity96T
Detection TargetShiga Toxins; Shiga Toxin; Shiga toxins 1 and 2; Stx1 and Stx2
Sample TypeFecal
ApplicationsELISA
ReactivityHuman

Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Website: https://www.creative-diagnostics.com/

(1) Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogensNature Biomedical EngineeringJuly 18, 2025Carlotta Ronda, Tyler Perdue, Logan Schwanz, Diego Rivera Rivera Gelsinger, Leonie Brockmann, Andrew Kaufman, Yiming Huang, Samuel H. Sternberg, Harris H. Wangusing an ELISA kit for simultaneous detection of Stx1 and Stx2 in a single test (Creative Diagnostics DEIASL162). For in vitro studies, pathogens were grown in LB with appropriate antibiotics shaking at 240 RPM at

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Shiga toxins ELISA Kit from Creative Diagnostics

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Shiga toxins ELISA Kit from Creative Diagnostics

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