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Shiga toxins ELISA Kit
Shiga toxins ELISA Kit from Creative Diagnostics
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Citations:
(1)
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Creative Diagnostics for
Shiga toxins ELISA Kit
Description
Qualitative
Product Specs
Company Info
Citations
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Product Specs
Item
Shiga toxins ELISA Kit
Company
Creative Diagnostics
Price
Supplier Page
View Company Product Page
Catalog Number
DEIASL162
Precision
Intra-Assay
For the determination of intra-assay performance, 6 positive fecal specimens and 6 negative fecal specimens were analyzed. Each specimen was assayed in replicates of eight. All positives remained positive and all negatives remained negative.
Inter-Assay
The inter-assay precision of the Shiga toxins ELISA Kit was determined using 12 fecal specimens (six negative, two positive for Stx1, two positive for Stx2, and two positive for both Stx1 and Stx2). The samples were tested, twice a day over a 5-day period using 2 different kit lots. A positive and negative control was run on each day. All positives remained positive and all negatives remained negative
Validation
The reproducibility of the SHIGA TOXIN CHEK test was determined using 11 fecal specimens that were coded to prevent their identification during testing. Testing was performed at 2 independent laboratories and on-site at TECHLAB
®
, Inc. The samples were tested, twice a day over a 5-day period by multiple technicians at each site using 2 different kit lots. A positive and negative control was run with each panel of the masked samples. The results from each laboratory were submitted to TECHLAB
®
, Inc. and compared with in-house results. The results were consistent among the different locations, and exhibited a correlation of 100%. The samples produced the expected results 100% of the time
Components
Microassay Plate –12 strips, each strip consisting of 8 wells, coated with monoclonal antibodies specific for Stx1 and Stx2 (stored with desiccant).
Diluent (40 mL) – buffered protein solution containing 0.02% thimerosal.
Substrate (14 mL) – solution containing tetramethylbenzidine and peroxide.
Wash Buffer Concentrate (50 mL) – 20X concentrate containing phosphate buffered saline, detergent, and 0.2% thimerosal.
Stop Solution (7 mL) – 0.6N sulfuric acid. CAUTION: Avoid contact with skin; flush with water immediately if contact occurs.
Positive Control (3.5 mL) – inactivated antigen in a buffered protein solution containing amphotericin B.
Conjugate (7 mL) – polyclonal antibodies specific for Stx1 and Stx2 coupled to horseradish peroxidase in a buffered protein solution containing 0.02% thimerosal
Sensitivity
The cutoff for the test for direct fecal specimens was established at concentrations of 0.28 ng/mL Stx1 and 0.23 ng/mL Stx2, and for broth cultures at concentrations of 0.18 ng/mL Stx1 and 0.30 ng/mL Stx2
Quantity
96T
Detection Target
Shiga Toxins; Shiga Toxin; Shiga toxins 1 and 2; Stx1 and Stx2
Sample Type
Fecal
Applications
ELISA
Reactivity
Human
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Creative Diagnostics
Phone
: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)
Email: info@creative-diagnostics.com
Company Profile
Website:
https://www.creative-diagnostics.com/
Citations (1)
(1)
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens
Nature Biomedical Engineering
July 18, 2025
Carlotta Ronda, Tyler Perdue, Logan Schwanz, Diego Rivera Rivera Gelsinger, Leonie Brockmann, Andrew Kaufman, Yiming Huang, Samuel H. Sternberg, Harris H. Wang
using an
ELISA kit
for simultaneous detection of Stx1 and Stx2 in a single test (
Creative Diagnostics
DEIASL162
). For
in vitro
studies, pathogens were grown in LB with appropriate antibiotics shaking at 240 RPM at
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Shiga toxins ELISA Kit from Creative Diagnostics
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