HEK293T HCP ELISA Kit DEIASL412 from Creative Diagnostics

Product Specs
ItemHEK293T HCP ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIASL412
Components1. Clear 96-Well Strip Plate
Clear plastic strip-well microtiter plate coated with rabbit anti-HEK293T HCP IgG. Can be used as individual strips.
2. HEK293T Protein Standard (1000 µg/ml, 50 µl)
Concentrated HEK293T proteins sufficient for generating a standard curve from 27 µg/ml to 37 ng/ml.
3. 5x Dilution Buffer (15 ml)
1xDilution Buffer is used for dilution of Reporting Antibody and Streptavidin-HRP conjugate. 1x Dilution Buffer is used to dilute samples if necessary. 10 ml of 5x concentrate should be diluted to 50 ml with 40 ml of milliQ water to achieve 1x Dilution Buffer.
4. 10x PBS-T (30 ml)
1xPBS-T is used for wash steps. 25 ml of 10x concentrate should be diluted to 250 ml with 225 ml of milliQ water to achieve 1x PBS-T.
5. Reporting Antibody (175 µl/tube)
A biotin labeled rabbit polyclonal antibody specific for HEK293T cell proteins. Three hours prior to the assay, dilute 150 µl into 15 ml of 1x Dilution Buffer.
6. Streptavidin-HRP Conjugate (40 µg/ml, 175 µl/tube)
A Streptavidin–Horse Radish Peroxidase conjugate in a stabilizing solution. Immediately prior to the assay, dilute 150 µl into 15 ml of 1x Dilution Buffer to give a 0.4 µg/ml working stock.
7. TMB Substrate (15 ml)
Use directly without dilution.
8. Stop Solution (15 ml)
A 1M solution of sulfuric acid. CAUSTIC. Use directly without dilution. The solution should not come in contact with skin or eyes. Take appropriate precautions when handling this reagent.
9. Plate Sealer
Quantity96T
Detection Range37-27000 ng/mL
Limit of quantification: 37 ng/mL
Detection TargetHEK 293 HCP
Sample Typebulk products
ApplicationsELISA

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Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

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Website: https://www.creative-diagnostics.com/

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HEK293T HCP ELISA Kit from Creative Diagnostics

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HEK293T HCP ELISA Kit from Creative Diagnostics

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