Monarch HMW DNA Extraction Kit for Cells & Blood T3050L from New England Biolabs

Product Specs
ItemMonarch HMW DNA Extraction Kit for Cells & Blood
CompanyNew England Biolabs
Price $420.00 Inquire
Catalog NumberT3050L
Temperature25 C
ApplicationsDNA Manipulation
Quantity50 preps
FormatBeads and tube
Sample TypeCultured cells and whole blood.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Nanopore-based sequencing of active DNA replication reveals key principles of metazoan replication dynamicsSci AdvMay 1, 2026Dongsheng Han, Cole Shepherd, Mary Lauren Benton, Jared T. Nordmanextraction and library preparation were conducted as a variation on New England Biolab’s adaption for their T3050L Kit for Oxford Nanopore Sequencing. EB+ was prepared by supplementing 1 ml of Monarch Elution Buffer

(2) Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing dataNucleic Acids ResApril 29, 2026T Rhyker Ranallo-Benavidez, Yue Hao, Emilia Volpe, Maryam Jehangir, Noelle Fukushima, Zachary D Stephens, Ogechukwu Mbegbu, Matteo T Ungaro, Rebecca Reiman, Jessica Molnar, et al.isolation using the NEB Monarch HMW DNA extraction kit for cells and blood (New England Biolabs, Cat. No. T3050L) following manufacturer’s instructions. HMW DNA was quantified by Nanodrop and Qubit™ 1xdsDNA High Sensitivity

(3) Population differences of chromosome 22q11.2 duplication structure predispose differentially to microdeletion and inversionNat CommunApril 18, 2026David Porubsky, DongAhn Yoo, Nidhi Koundinya, Erika Souche, Philip C. Dishuck, Nicolas Dierckxsens, William T. Harvey, Katherine M. Munson, Kendra Hoekzema, Daniel D. Chan, et al.cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). At all steps, quantification was performed with Qubit dsDNA HS (Thermo Fisher, Q32854), measured on

(4) Patient-derived organoid xenografts reveal the multifaceted role of the lncRNA MALAT1 in breast cancer progressionbioRxivApril 3, 2026Disha Aggarwal, Suzanne Russo, Kendall Anderson, Taylor Floyd, Raditya Utama, James A. Rouse, Payal Naik, Sara Pawlak, Shruti V. Iyer, Melissa Kramer, et al.from 50-100 μl of thawed organoid pellets using the ‘Extraction from Cells’ Monarch kit (NEB Catalog #T3050) and quantified using the Qubit fluorometer (Thermo Fisher Scientific) and integrity was assayed using

(5) MASTR-seq enables multiplexed analysis of short tandem repeats with sequencingCell Rep MethodsMarch 23, 2026Chuanbin Su, Han-Seul Ryu, Keerthivasan Raanin Chandradoss, Thomas Malachowski, Ravi Boya, Linda Zhou, Hoa Emma Nguyen, Esteban O. Mazzoni, Kristen J. Brennand, Jennifer E. Phillips-Creminsquality assay We obtain HMW DNA from 5-10×106 iPSCs or 20 mg brain tissue with commercial kits (NEB #T3050S/3060S). Use a broad range dsDNA quantitation kit to detect HMW genomic DNA concentration. Assess gDNA

(6) The tiger who came to T2T: Telomere-to-Telomere genome assembly of the Sumatran tiger (Panthera tigris sumatrae) using nanopore simplex readsBMC GenomicsMarch 21, 2026Laura Louise Dean, Nadine Holmes, Phillipa Dobbs, Matthew Looseextracted from the blood sample using the Monarch HMW DNA Extraction Kit for Cells and Blood (NEB; T3050L), using the Frozen Blood Extraction section of the instruction manual (NEB; www.neb.com/T3050). Samples

(7) Distinct mechanisms of CNV formation at the human 15q13.3 locusbioRxivMarch 5, 2026Wolfram Höps, David Porubsky, DongAhn Yoo, Michelle de Groot, Amber den Ouden, Ronny Derks, Kendra Hoekzema, Alexander Hoischen, Helger G. Yntema, Human Pangenome Reference Consortium (HPRC), et al.extracted from frozen blood samples using the NEB Monarch HMW DNA extraction kit for Cells & Blood (#T3050L) following the manufacturer’s protocol with the following exceptions: 500 uL blood was used for the

(8) Impact of Tau overexpression on DNA replication dynamics in centromeres of human neural progenitor cellsiScienceFebruary 20, 2026Elisa Balzano, Shyam Twayana, Settapong T. Kosiyatrakul, Glennis A. Logsdon, Bhushan L. Thakur, Evan E. Eichler, Bettina Bohl, Philipp Koch, Simone Sidoli, Anupama Kumari, et al.extracted from cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs P/N T3050L). Then, the HMW DNA was used to generate PacBio HiFi libraries via the SMRTbell prep kit 3.0 and Barcoded

(9) Using the linear references from the pangenome to discover missing autism variantsNature CommunicationsJanuary 23, 2026Yang Sui, Jiadong Lin, Michelle D. Noyes, Youngjun Kwon, Isaac Wong, Nidhi Koundinya, William T. Harvey, Mei Wu, Kendra Hoekzema, Katherine M. Munson, et al.trios, DNA was extracted from blood using the Monarch HMW DNA Extraction Kit for Cells & Blood from NEB (T3050L) (n = 6) or the Qiagen Puregene Blood Core Kit (158023) (n = 9), following the manufacturer’s specifications.

(10) Automated mapping of DNA replication fork progression in human cells with ForkMLNature CommunicationsJanuary 23, 2026Victoria Rojat, Diletta Ciardo, Alan Tourancheau, Florence Proux, Etienne Jean, Jean-Michel Arbona, Benjamin Audit, Gael A. Millot, Frédéric Bonhomme, Paola B. Arimondo, et al.genomic DNA was extracted using Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs #T3050) according to the manufacturer’s instructions for ultra-long DNA sequencing applications. Conditioned

(11) Antisense oligonucleotides to KIF1A polymorphisms expand targets and rescue patient-derived neurons in vitroNature CommunicationsJanuary 9, 2026Michael V. Zuccaro, Randee E. Young, Jiangyuan Hu, Patricia Lanzano, Ekaterina Semenova, Xiaoqi Lin, Alicja Skowronski, Yufeng Shen, Tae Hyun Kim, Danny E. Miller, et al.extracted from whole blood using New England Biolabs Monarch® HMW DNA Extraction Kit for Cells & Blood (T3050L). DNA was also extracted using Puregene Blood Kit (Qiagen 158023) according to the manufacturer instructions. Extracted

(12) Satellite DNA fragility accompanies complex genome rearrangements and ecDNA oncogene amplification in canine osteosarcomasbioRxivJanuary 3, 2026Feyza Yilmaz, Wonyoung Kong, Sabriya A. Syed, Francis H. O’Neill, Jody T. Lombardi, Patrick Kwok Shing Ng, Ching C. Lau, Charles Leewhole genome sequencing Genomic DNA (gDNA) was extracted from blood and tissue samples using NEB Monarch cell/blood (NEB #T3050) and NEB Monarch tissue (NEB #T3060) kits, respectively. Briefly, 0.5-1ml of blood was mixed with 3

(13) Intra-strain genetic heterogeneity in Toxoplasma gondii ME49: Oxford Nanopore long-read sequencing reveals copy number variation in the ROP8-ROP2A locusBMC GenomicsDecember 3, 2025Yomna Gohar, Marie Neumann, Lisanna Hülse, Daniel Wind, Julia Mock, Karin Buchholz, Marcel Helle, Ursula R. Sorg, Daniel Degrandi, Klaus Pfeffer, et al.non-fragmented DNA for genome assembly (Supplementary Table 1). Monarch HMW DNA Extraction Kit (NEB, Cat# T3050L): DNA was extracted using the Monarch HMW DNA Extraction Kit according to the manufacturer’s protocol.Size

(14) Cell line-matched reference enables high-precision functional genomicsNature CommunicationsNovember 20, 2025Luca Corda, Emilia Volpe, Hamza Dallali, Elena Di Tommaso, Alessio Colantoni, Andrea Guarracino, Sai Swaroop Chittoor, Mattia Capulli, Evelyne Tassone, Simona Giuntacell pellets using the Monarch HMW DNA extraction kit for cells and blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L). HMW DNA was quantified by Nanodrop and Qubit with a broad range kit (Thermo Scientific

(15) A mammalian-like piRNA pathway in Axolotl reveals the origins of piRNA-directed DNA methylationThe EMBO JournalNovember 13, 2025Xinyu Xiang, Anni Gao, Dominik Handler, Francisco Falcon, Diego Rodriguez-Terrones, Sergej Nowoshilow, Wanlu Liu, Elly M Tanaka, Dónal O’Carrollextracted from three sperm samples of the white axolotl strain using Monarch HMW DNA Extraction Kit (NEB, T3050L) and solubilized in 10 mM Tris, pH 9.0, 0.5 mM EDTA. Methyl-seq libraries were prepared using the NEBnext Enzymatic Methyl-seq kit

(16) Variation in surface protein expression leads to heterogeneous Trypanosoma cruzi populations during host cell infectionNature CommunicationsNovember 12, 2025Lissa Cruz-Saavedra, Mira Loock, Luiza Berenguer Antunes, Igor CestariNa2HPO4, 1.47 mM KH2PO4), resuspended in 200 μL of lysis buffer from NEB Monarch HMW DNA Extraction (NEB #T3050, Massachusetts, USA), and processed following the manufacturer’s protocol. The DNA integrity was verified

(17) Complete genome assemblies of two mouse subspecies reveal structural diversity of telomeres and centromeresNature GeneticsNovember 1, 2025Bailey A. Francis, Landen Gozashti, Kevin Costello, Takaoki Kasahara, Olivia S. Harringmeyer, Jingtao Lilue, Tianzhen Wu, Katarzyna Zoltowska, Mohab Helmy, Tadafumi Kato, et al.extracted from 1 million (PacBio) to 5 million (Oxford Nanopore - ONT) cell pellets using the Monarch T3050 kit and protocol (New England Biolabs). For ONT ultralong, recommended protocol adjustments were carried

(18) Young KRAB-zinc finger gene clusters are highly dynamic incubators of ERV-driven genetic heterogeneity in miceNat CommunOctober 30, 2025Melania Bruno, Sharaf M. Farhana, Apratim Mitra, Kevin Costello, Dawn E. Watkins-Chow, Glennis A. Logsdon, Craig W. Gambogi, Beth L. Dumont, Ben E. Black, Thomas M. Keane, et al.Ultra-HMW genomic DNA was extracted with the Monarch HMW DNA Extraction Kit for Cells and Blood (NEB T3050) following manufacturer instructions. Libraries were prepared using the Nanopore Ultra-Long Sequencing

(19) Direct identification of de novo mobile element insertions from single molecule sequencing of human spermbioRxivOctober 29, 2025Stacy Li, Kenneth Aston, Joseph G Gleeson, Aaron Quinlan, Xiaoyu Yang, Peter H Sudmantmodified version of the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs Cat. #T3050L) extraction protocol. Cell lysis solution (100 uL of Nuclei Prep Buffer, 100 uL of Lysis Buffer, 15

(20) Evidence for improved DNA repair in the long-lived bowhead whaleNatureOctober 29, 2025Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, et al.DNA from human primary fibroblasts was with the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB T3050L). To produce the damaged DNA samples and induce PAR formation, cells were treated with H2O2 and UV prior

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Monarch HMW DNA Extraction Kit for Cells & Blood

Monarch HMW DNA Extraction Kit for Cells & Blood from New England Biolabs

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Monarch HMW DNA Extraction Kit for Cells & Blood from New England Biolabs

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