Fig 1: Genetic association with MTIL and T/NK cell levels.a, MTIL expression in adnexal malignant cells (Discovery and Test 2 datasets, n = 264,825 cells) residing in tissue niches where T/NK cells were not detected, stratified by tissue profiles labeled by patient and dataset. Dashed brackets indicate adnexal malignant cells from patient-matched tissue profiles from Discovery and Test 2. b, MTIL expression in malignant cells (Discovery dataset), stratified by somatic copy number of six respective genes based on patient-matched bulk tumor genomic profile (LMM, n = 40 patients). c, Top CNAs showing a significant (BH FDR < 0.05, LMM; Methods) association with MTIL expression in malignant cells in the Discovery dataset. d, CNA–MTIL–TIL models. e, Deletion (red) of MTIL-up genes and amplification (light blue) of MTIL-down genes that are significantly (BH FDR < 0.05, one side t-test) associated with low T/NK cell levels (estimated based on gene expression of T/NK cell signatures; Methods) in an independent TCGA HGSC cohort of 578 patients12. Exact P values are provided in Supplementary Table 9a. f, ELISA quantification of IFN-γ (1:100) and TNF (undiluted) in NK-92 supernatant treated with various concentrations of recombinant human BMP7. g, MTIL chemokines and the matching chemokine receptors in immune cell TIPs. h, Fold change of NK-92 cell migration of CXCR6+ NK-92 cells derived via CRISPR activation (Supplementary Fig. 9) versus control NK-92 cells (transduced with non-targeting control CRISPR activation guides, left) at varying CXCL16 concentrations. In the box plots in a and b, the middle line denotes the median, box edges indicate the 25th and 75th percentiles, and whiskers extend to the most extreme points that do not exceed ±1.5 times the IQR; further outliers are marked individually with circles (minima/maxima). In f and h, error bars represent the mean ± s.d. for f and mean ± s.e.m. for h; comparisons are indicated via brackets; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (ordinary one-way ANOVA); brackets that are not shown denote nonsignificant (P > 0.05) comparisons. Data from n = 3 biological replicates were collected per condition in f and n = 4 technical replicates were collected per condition in h.Source data