Fig 1: OLFM4 alleviated mitochondrial dysfunction in lung epithelial cells. (A) MLE-12 cells were transfected with OLFM4 plasmids or NC for 24 h before LPS stimulation. ROS production in MLE-12 cells was evaluated using a microplate reader. (B) The expression of HIF-1α and phosphorylated LDHA (p-LDHA) in MLE-12 cells was detected by Western blot. (C) BEAS-2B cells were pre-treated with human recombinant OLFM4 for 30 min before stimulated with LPS. ROS production in BEAS-2B cells was evaluated using a microplate reader. (D) The expression of HIF-1α and phosphorylated LDHA (p-LDHA) in BEAS-2B cells was detected by Western blot. **p < 0.01 versus CT group, ##p < 0.01 versus LPS group.
Fig 2: HIF1α is involved in the regulation of OLFM4 in liver cancer proliferation. (a) siRNAs against HIF1α successfully inhibited the mRNA expression of HIF1α in HepG2 cells measured by qRT-PCR assay ( ∗∗P < 0.01, ∗∗∗P < 0.001); (b) the knockdown of HIF1α alleviated the increase of OLFM4 plasmid on liver cancer proliferation in HepG2 cells.
Fig 3: Correlation of OLFM4 to tumor size. (a) Correlation analysis about BMI to tumor size; (b) correlation analysis about OLFM4 blood level to tumor size; and (c) correlation analysis about mRNA expression of OLFM4 in HCC tissue to tumor size.
Fig 4: OLFM4 expression was different between septic and sepsis-induced ARDS patients. (A and B) Heatmap and statistical analyses of expression of the ten hub genes in the GSE66890 dataset. Red = upregulated. Blue = downregulated. **p < 0.01. (C) Analyses of ROC curves of critical DEGs in the GSE66890 dataset. ROC curves were generated and the area under the ROC was used to compare the ten genes in the sepsis group and sepsis-related ARDS group. Three DEGs showed AUC >0.7: 0.716 for OLFM4, 0.719 for LCN2, and 0.735 for BPI.
Fig 5: OLFM4 expression in lung epithelial cells was induced by neutrophil media. (A) MLE-12 cells were incubated with LPS (5 µg/mL) for 6, 12, 24, and 48 h, and the expression level of OLFM4 was detected by Western blot. (B) MLE-12 cells were incubated with different doses of LPS (500 ng/mL, 1 µg/mL, 5 µg/mL, 10 µg/mL) for 24 h, and OLFM4 expression was measured by Western blot. (C) BEAS-2B cells were incubated with LPS (5 µg/mL) for 6, 12, 24, or 48 h, and OLFM4 expression was measured by Western blot. (D) Murine BMDNs were purified and the purity was assessed by flow cytometry. (E) BMDNs were stimulated with or without LPS for 4h, and BMDN media was collected. MLE-12 cells were treated with BMDN media or DMEM and OLFM4 expression was measured by Western blot.
Supplier Page from Abcam for Human OLFM4 ELISA Kit