Fig 1: Effects of anti-HIV drugs on expression of selective factors related to hepatic metabolism or secretion of bile acid or bilirubin. (A) Western blots of proteins from HepG2; Ctrl, non-senescent HepG2; Senescent, senescent HepG2; DMSO, dimethyl sulfoxide as vehicle control; RTV, ritonavir; LPV, lopinavir; RTV + LPV, ritonavir plus lopinavir; OPTP1B1 and OATPIB3, organic anion transporter polypeptide member 1B1 and 1B3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase as an internal control; UGT1A1, UDP-glucuronosyltransferase (UGT) 1A1; (B–D), RT-PCR of mRNAs from HepG2; CYP3A4, CYP2D6, and CYP7A1: cytochrome P450 3A4, 2D6, and 7A1, respectively; Green dots indicate drug treatments; **, p < 0.01; ***, p < 0.005; (E) Enzyme activities of UGT from non-senescent PHHs (Ctrl) or senescent PHHs (Senescent); *, p < 0.05; **, p < 0.01 compared to Ct of the same cell group; δ, p < 0.05 and δδ, p < 0.01 compared to the same treatment between non-senescent and senescent PHHs.