CUT&RUN Assay Kit 86652S from Cell Signaling Technology

Product Specs
ItemCUT&RUN Assay Kit
CompanyCell Signaling Technology
Price $557.00 Inquire
Catalog Number86652S
Quantity1 Kit
Research AreaChromatin Regulation / Nuclear Function
ApplicationsC&R

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Prolonged KRAS-MAPK Inhibition Induces Interferon Signaling that Promotes Cell State Transition and Confers Therapeutic VulnerabilitiesCancer ResApril 20, 2026Ashenafi Bulle, Yali Chen, Huaping Li, Timothy Hung-Po Hung-Po Chen, Iftikhar Ali Khawar, Lin Li, Yu Wang, Peng Liu, Vikas Kumar Somani, Richard Kurupi, et al.factors in Pa01C pancreatic cancer cells using the CUT&RUN Assay Kit (Cell Signaling Technology, Cat# 86652S), following the manufacturer’s instructions. Cells were seeded at a density of 1 × 104 cells/cm2 in

(2) PHF2 regulates grip strength via demethylation at the promoter region of the Mef2ciScienceApril 17, 2026Taku Fukushima, Taiju Fujioka, Yuuki Imai, Satoru Masubuchi, Iori Sakakibarawas performed on GA muscles from 4-week-old male mice according to the CUT&RUN Assay Kit protocol (86652, CST). GA muscles were not cross-linked. Tissues were minced with scissors, suspended in wash buffer,

(3) Inhibiting LSD1 unlocks retinoid AP-1 programming to activate epithelial immunity and skin tumor suppressionJournal of Clinical InvestigationApril 15, 2026Nina Kuprasertkul, Alyssa F. Moore, Carina A. D'souza, Julia Chini, Eun-Kyung Ko, Sijia Huang, Shuo Zhang, Ashley S. Anderson, Shaun Egolf, Laura V. Pinheiro, et al.Countess). CUT&RUN. H3K4me2 CUT&RUN was performed using the CUT&RUN Assay Kit (Cell Signaling Technology, 86652S). A total of 200,000 cells from either WT or LSD1-KO mice were used. Antibodies included H3K4me2 (Abcam

(4) H3 dopaminylation and CaMKII modulate diffuse midline glioma response to CDK9 inhibitionbioRxivApril 10, 2026Rebecca L. Murdaugh, Brittany R. Eberl, Rosemary U. Richard, Ena I. Campos-Hensley, Adanze N. Nnyagu, W. Austin Elam, Ai-Ni Tsao, Jack R. Tremblay, Ruiying Ma, Alfred K. Dei-Ampeh, et al.cells/sample. CUT&RUN-enriched DNA was prepared using the Cell Signaling Technologies CUT&RUN Kit (cat# 86652) following the manufacturer’s protocol using the following antibodies: pS2-Pol2 (cat# 04–1571, MilliporeSigma),

(5) Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein responseBioact MaterApril 9, 2026Yu Sun, Qifeng Chen, Yan Zhao, Yuanyuan Yin, Qiao Chen, Lixin Yang, Zixuan Liu, Rui Chen, Xiaohui Xu, Jinlin Songusing nuclease (CUT&RUN) assays were performed in biological duplicates using the CUT&RUN Assay Kit (86652, CST). Briefly, cells were treated with Trypsin-EDTA (25300062, Gibco) for 60 s, and 250,000 cells were

(6) Friend of GATA2 Variant Ser657Gly Is Associated With Coronary Microvascular DiseaseCirc Genom Precis MedApril 1, 2026Marie A. Guerraty, Shefali S. Verma, Yi-An Ko, Michael A. McQuillan, Donna M. Conlon, John W. Tobias, Michael G. Levin, William Haury, Fangyuan Zheng, Tess Cherlin, et al.Cleavage Under Target and Release Under Nucleosome (CUT&RUN) kit (Cell Signalling Technology [CST] no. 86652) was performed using HIF1a (hypoxia-inducible factor; CST no. 36169S and Abcam no. 2185) or control

(7) CBP-IDRs regulate acetylation and gene expressionCell RepMarch 24, 2026Katie L. Gelder, Nicola A. Carruthers, Grace Gilbert, Laura J. Harrison, Brychan V. Evans, Thomas I. Evans, Sophie S. Ball, Mark Dunning, Timothy D. Craggs, Alison E. Twelvetrees, et al.Illumina,NEB,E7645 TapeStation High Sensitivity D1000 ScreenTape,Agilent,5067–5584 CUT&RUN Assay kit,Cell Signaling,86652S Quant-seq 3′ mRNA-Seq Library Prep kit,Lexogen,191.24 qPCR Add-on Kit,Lexogen,208.96 ChromoTek GFP-Trap® Magnetic Particles M-270

(8) Persistent viral control status is associated with enhanced innate immune responses in people with HIV-1iScienceMarch 20, 2026Jéssica C. dos Santos, Albert L. Groenendijk, Suzanne D.E. Ruijten, Rainer Knoll, Nadira Vadaq, Rob ter Horst, Ezio T. Fok, Wojciech Witkowski, Marc J.T. Blaauw, Louise E. van Eekeren, et al.Infinium iSelect 24x1 HTS Beadchip kit,Illumnia,Cat#WG-405-1014 CUT&RUN Assay kit,Cell Signaling,Cat#86652 KAPA HyperPrep Kit,Roche,Cat#KK8502 High Sensitivity DNA Kit for 2100 Bioanalyzer Systems,Agilent,Cat#5067 Olink®

(9) Foxn3 is part of a transcriptional network that regulates primary cilia in the developing retinaDevelopmentMarch 2, 2026Huanqing Zhang, Thejas Nair, Fan Meng, David L. Turnereach CUT&RUN assay, which were performed using the CUT&RUN Assay Kit (Cell Signaling Technologies, CST 86652S) following the manufacturer’s instructions for live cell preparation with modifications: micrococcal

(10) An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progressioniScienceFebruary 20, 2026Amber J. Kiliti, Ghada M. Sharif, Megan E. McNamara, Raneen Rahhal, Susan Prewitt, Marcel O. Schmidt, Ci Wu, Junfeng Ma, Eric Glasgow, Anton Wellstein, et al.Cell populations were then separated via FACS and the CUT&RUN Assay Kit (Cell Signaling Technology, 86652P) was performed as above. Data analysis TrimGalore (v0.6.6) was used to trim paired-end reads to remove

(11) Epigenetic and Transcriptional Programs Define Osteosarcoma Subtypes and Establish Targetable VulnerabilitiesCancer DiscoveryFebruary 6, 2026Eunice López-Fuentes, Andrew S. Clugston, Alex G. Lee, Leanne C. Sayles, Natalie Sorensen, Maria V. Pons Ventura, Stanley G. Leung, Truc Dinh, Marcus R. Breese, E. Alejandro Sweet-Corderocells. TF Chromatin Binding CUT&RUN was performed using the CUT&RUN Assay Kit (Cell Signaling Technology, #86652) according to the manufacturer’s protocol. Per antibody, 1.0 × 105 cells were used. Briefly, cells were

(12) Oncogenic KRAS/ERK/JUNB signaling suppresses differentiation regulator GATA6 in pancreatic cancerJournal of Clinical InvestigationFebruary 2, 2026Zheng Zhong, Xinang Cao, Pei-Ju Liao, Raman Sethi, Jeffrey A. Klomp, Clint A. Stalnecker, Jinmiao Chen, Yue Wan, Channing J. Der, David M. Virshupassay for JUNB was performed in HPAF-II cells using the CUT&RUN Assay Kit (Cell Signaling Technology, 86652) following the manufacturer’s protocol. 200,000 untreated cells or cells treated with DMSO control or

(13) Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanismJournal of Biological ChemistryFebruary 1, 2026Jason L. Larabee, Elizabeth J. Donald, Anushka A. Sukhadia, Tyler M. Shadid, Sarah J. Miller, Jimmy D. Ballardsites for CTCF were isolated using CUT&RUN reagents obtained from Cell Signaling Technology (product # 86652). Antibodies used for this assay include a rabbit monoclonal antibody against CTCF (Cell Signaling Technology;

(14) Multi-Omics Analysis of Human Blood Cells Reveals Unique Features of Age-Associated Type 2 CD8 Memory T CellsAging CellFebruary 1, 2026Hiroyuki Matsui, Marlene Cervantes, Mir M. Khalid, Alan Tomusiak, Varun Dwaraka, Jorge Landgrave-Gomez, Prasanna Vadhana Ashok Kumaar, Qingwen Chen, Jessica Lasky-Su, Jake Stone, et al.were made to the manufacturer's protocol for the CUT & RUN Assay Kit (Cell Signaling Technologies, #86652). Briefly, live cells were washed (+spermine, +PIC) and mixed with activated Concanavalin A beads. After

(15) Hypoxic stress is an early pathogenic event in human VCP-mutant ALS astrocytesStem Cell ReportsJanuary 13, 2026Hannah D. Franklin, Hamish Crerar, Nishita Parnandi, Michael Lattke, Stanislaw Majewski, Benjamin E. Clarke, Husayn Pallikonda, Michael Howell, Simon J. Boulton, Rickie Pataniwith 500 μM DMOG for 24 h, lightly fixed, and processed using the Cell Signaling CUT&RUN assay kit (#86652) according to the manufacturer’s instructions, followed by Illumina-compatible library preparation and

(16) SOX4-STAT6-MTHFD2 axis drives hepatocellular carcinoma progression and treatment resistanceCell Death & DiseaseJanuary 3, 2026Chia-Lung Tsai, Ming-Chin Yu, Cheng-Lung Hsu, Hsiang-Yu Tang, Yun-Shien Lee, Lang-Ming Chi, Sey-En Lin, Mei-Ling Cheng, Heng-Yuan Hsu, Chi-Neu Tsaiin Supplementary Table 5. Chromatin immunoprecipitation (ChIP) sequence CUT&RUN (Cell Signaling, 86652) was performed on 1 × 10⁵ Hep3B or Hep3B SOX4−/− cells with anti-SOX4 (Abnova, PAB14092). SOX4–DNA complexes

(17) Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancerFEBS LettersJanuary 1, 2026Diego Claro de Mello, Marcella Maringolo Cristovão, Guilherme Henrique, Vinicius Gonçalves Rodrigues, Caroline Serrano‐Nascimento, Edna Teruko Kimura, Cesar Seigi Fuziwaraunder Targets and Release Using Nuclease (CUT&RUN) assay was performed using the CUT&RUN Assay Kit (#86652) from Cell Signaling Technologies. One hundred thousand SW1736 cells per immunoprecipitation (IP), including

(18) Targeting Wnt/β‐Catenin Pathway by DNA Alkylating Pyrrole‐Imidazole Polyamide in Colon CancerCancer ScienceJanuary 1, 2026Osamu Shimozato, Natsue Akao, Yoko Yanagisawa, Yusuke Mori, Xudong Zhang, Yuki Kida, Rie Igarashi, Takayoshi Watanabe, Toshinori Ozaki, Atsushi Takatori(CUT&RUN) Assay CUT&RUN [36] was performed using a commercially available kit (CUT&RUN assay kit, #86652, Cell Signaling Technology, Beverly, MA, USA). Briefly, HCT116 cells (100,000 cells/target protein)

(19) Neural crest induction requires SALL4-mediated BAF recruitment to lineage specific enhancersDevelopment (Cambridge, England)December 15, 2025Demurtas M, Barnada SM, van Domselaar E, Mitchell ZH, Deelen L, Trizzino M.http://www.graphpad.com/faq/viewfaq.cfm?faq=1362). CUT&RUN For each condition, 1×105 cells were processed using CUT&RUN Assay Kit (Cell Signaling Technology, #86652). Briefly, each sample was resuspended in Concanavalin A Beads and treated with an Antibody Binding

(20) Transcriptional interferences ensure one olfactory receptor per ant neuronNatureDecember 11, 2025Bogdan Sieriebriennikov, Olena Kolumba, Aurore de Beaurepaire, Jennifer Wu, Valentina Fambri, Eva Bardol, Yuwei Zhong, Ildar Gainetdinov, Danny Reinberg, Hua Yan, et al.for 10x Single Cell 3' kit,PacBio,Cat#102-659-600 CUT&RUN Assay Kit,Cell Signaling Technology,Cat#86652 NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads,New England Biolabs,Cat#E7870 Deposited

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CUT&RUN Assay Kit from Cell Signaling Technology

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CUT&RUN Assay Kit from Cell Signaling Technology

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