NEBNextrRNA Depletion Kit v2(Human/Mouse/Rat) E7400S from New England Biolabs

Product Specs
ItemNEBNextrRNA Depletion Kit v2(Human/Mouse/Rat)
CompanyNew England Biolabs
Price $303.00 Inquire
Catalog NumberE7400S
Quantity6 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Olivetol induces a non-genotoxic nucleolar DNA damage response via membrane-dependent stress signalingNucleusDecember 31, 2026Dmitry A. Deriglazov, Nadezhda V. Petrova, Artem V. Luzhin, Nataliya G. Pavlenko, Victor V. Tatarskiy, Eugene P. Kazakov, Igor I. Kireev, Alexander V. Kurkin, Anton M. Novoselov, Yuri M. Efremov, et al.for sequencing were prepared using NEBNext® rRNA Depletion Kit v2 (Human/mouse) (New England Biolabs, E7400, E7405) according to the manufacturer’s protocol. Next generation sequencing (NGS) was performed on

(2) TCA cycle rewiring underpins histone acetylation sourcing and cell-fate transitions during exit from naive pluripotencyCell Stem CellMay 7, 2026Eleni Kafkia, David Pladevall-Morera, Lidia Argemi-Muntadas, Gangqi Wang, Roberta Noberini, Arnau Casòliba-Melich, Sandra Bagés-Arnal, Matthias Anagho-Mattanovich, Rita Silvério-Alves, Johanna Gassler, et al.for Illumina®,New England Biolabs,Cat# E7765 NEBNext rRNA Depletion Kit v2,New England Biolabs,Cat# E7400S NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 2,New England Biolabs,Cat# E7780S Qubit dsDNA HS Assay Kit

(3) The Roles of MKI67, GNL2, and MDN1 in Ribosome Biogenesis and Transcriptome Regulation in the Neuronal Cell Line HEK293TbioRxivMay 5, 2026Shiro Iuchi, Joao A. Paulototal RNA (1000 ng) was subjected to ribosomal RNA depletion using the NEBNext rRNA Depletion Kit v2 (E7400S), and the RNA sample was purified using NEBNext Sample Purification Beads (E7767S). Fragmentation was

(4) C9orf72 hexanucleotide repeat RNA drives transcriptional dysregulation through genome-wide DNA:RNA hybrid G-quadruplexesNeuronMarch 18, 2026Honghe Liu, Mingming Liu, Yang Liu, Gege Gui, Tapas Paul, Yu-Ning Lu, Zhiyuan Huang, Haocheng Wang, Yatao Xiao, Zhongfan Zheng, et al.II RNA Library Prep Kit for Illumina (NEB, E7770) coupled with NEBNext rRNA Depletion Kit v2 (NEB, E7400). ATAC-seq ATAC-seq was conducted as previously described7 with minor modifications. Cells were freshly

(5) Mitochondrial control of glycerolipid synthesis by a PEP shuttleCellMarch 17, 2026Tadashi Yamamuro, Daisuke Katoh, Guilherme Martins Silva, Hiroshi Nishida, Satoshi Oikawa, Yusuke Higuchi, Dandan Wang, Masanori Fujimoto, Naofumi Yoshida, Mark Li, et al.XT DNA Library Prep Kit,Illumina,Cat# FC-131 NEBNext rRNA Depletion Kit v2,New England Biolabs,Cat# E7400X NEBNext mRNA Second Strand Synthesis Module,New England Biolabs,Cat# E6111L "NucleoSpin Plasmid EasyPure,

(6) Integration of Short- and Long-Read RNA Sequencing Enables the Discovery of Circular RNAsCancer ResMarch 2, 2026Sidi Z. Swift, Jace Webster, Hung Mai, Ghofran Othoum, Li Lin, Amy Ly, Emily B. Rozycki, Jacqueline L. Mudd, Emily Coonrod, Ashna Agarwal, et al.sequencing with the following exceptions. We used the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (#E7400X, New England BioLabs, Ipswich, MA) to remove the rRNA. Per the manufacturer’s protocol, multiple reactions

(7) MYC-ATF4-ASS1 axis governs intracellular arginine synthesis and dictates the immune microenvironment in melanomabioRxivMarch 2, 2026Haiwei Mou, Veronika Yakovishina, Yeqing Chen, Min Xiao, Maggie Dunne, Nancy Shi, Monzy Thomas, Kristen DeRosa, Haiyin Li, Qin Liu, et al.using the RNeasy Plus Mini Kit (Qiagen, C74134). We then used the NEBNext® rRNA Depletion Kit v2 (NEB, E7400) to deplete ribosome RNA (rRNA) and NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB, 7760)

(8) NELF prevents transcriptional readthrough into DNA replication zones in cancer cellsEMBO ReportsFebruary 20, 2026Chihiro Nakayama, Qi Fang, Yasukazu Daigaku, Yuki Aoi, Shoko Ito, Mami Takahashi, Reo Shimatani, Tamiko Minamisawa, Yagiz Ozturk, Hiroshi Kimura, et al.C10633 NEBNext Magnesium RNA Fragmentation Module,NEB,Cat# E6150 NEBNext rRNA Depletion Kit v2,NEB,Cat# E7400 NEBNext Ultra II DNA Library Prep Kit for Illumina,NEB,Cat# E7645 Blood & Cell Culture DNA Midi Kit

(9) Characterising processing conditions that artifactually bias human brain tissue transcriptomesNature CommunicationsFebruary 17, 2026Moein Yaqubi, Michael Thomas, Jonathan Talbot-Martin, Abdulshakour Mohammadnia, Alexis Allot, Adam M. R. Groh, Nurun Fancy, Marianna Papageorgopoulou, Xiaowen Zhang, Aisling McGarry, et al.concentration and underwent ribodepletion using the New England Biolabs rRNA depletion kit (cat no E7400X). Genomic Libraries were constructed using the New England Biolabs Library construction kit (cat no

(10) Human CRAMP1 specifically promotes the expression of histone H1 genesEMBO ReportsFebruary 9, 2026Justin Bodner, Pranathi Vadlamani, Alexander S Lee, Kathryn A Helmin, Qianli Liu, Almira E Pratasenia, Maria M A Horst, Sudharsana Ravisankar, Sakshi Khurana, Marc L Mendillo, et al.TRIzol reagent and chloroform. Ribosomal RNA was depleted with NEBNext rRNA Depletion Kit V2 (NEB, E7400) as per the manufacturer’s protocol. The rRNA-depleted samples were used as input for Library Prep as

(11) Identification of enzymatically modified isoquercitrin as a therapeutic lead for myotonic dystrophy type 1NAR Molecular MedicineJanuary 29, 2026Subodh K Mishra, Sawyer M Hicks, Jesus A Frias, Jing Zhang, Mark T Handley, Sweta Vangaveti, Tammy S Reid, Aradhana Pandey, Omari McMichael, Christopher Crumbaugh, et al.Directional RNA Library Prep Kit for Illumina (NEB #E7760) with NEBNext rRNA Depletion Kit v2 (NEB #E7400) was used to prepare RNAseq libraries, with a total of 1000 ng of RNA input for each library. The manufacturer’s

(12) DNA methylation and lncRNA control asynchronous DNA replication at specific imprinted gene domainsNature CommunicationsJanuary 21, 2026Yui Imaizumi, François Charon, Caroline Surcis, Christel Picard, Pol Arnau-Romero, Jean-Christophe Andrau, Daan Noordermeer, Benoit Moindrot, Jean-Charles Cadoret, Robert Feil#AMPD1) and ribodepletion with NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) (New England Biolabs, E7400S). RNA was then processed with Next® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs,

(13) HIV-1 transcription dominates over host gene activity at the HIV-1 integration sitemBioJanuary 14, 2026Samuel Weissman, Yang-Hui J. Yeh, Miriam Viazmenski, Rachel Kim, Jack A. Collora, Savannah Steinhauser, Ya-Chi HoIllumina for RNA-sequencing (NEB #E7760) for library prep with NEBNext rRNA Depletion Kit v2 (NEB #E7400). cDNA libraries were sequenced on NovaSeq 6000. Bioinformatic analysis of ATAC-seq and RNA-seq To

(14) Therapeutic remodeling of the ceramide backbone prevents kidney injuryCell MetabolismJanuary 6, 2026Rebekah J. Nicholson, Luis Cedeño-Rosario, J. Alan Maschek, Trevor Lonergan, Jonathan G. Van Vranken, Angela R. S. Kruse, Chris J. Stubben, Liping Wang, Deborah Stuart, Queren A. Alcantara, et al.ng) were hybridized with NEBNext rRNA Depletion Kit v2 (Human, Mouse, Rat) (New England Biolabs, Cat#E7400) to substantially diminish rRNA from the samples. Stranded RNA sequencing libraries were prepared using

(15) Topoisomerase III-beta protects from immune dysregulation and tumorigenesisSci AdvDecember 19, 2025Md Rasel Al Mahmud, Simone Andrea Baechler, Anjali Dhall, Sourav Saha, Hongliang Zhang, Laura Bassel, Shuling Zhang, Min-Jung Lee, Nahoko Sato, Shraddha Rastogi, et al.9.0. Library was prepared for splenocytes using the NEBNext rRNA Depletion Kit v2 (human/mouse/rat) (NEB, #E7400) and the NEBNext Ultra II Directional RNA Library Prep Kit from Illumina (NEB, #E7760). For polymerase chain reaction (PCR

(16) RBProximity-CLIP Enables Subcellular Mapping of RNA–Binding Protein Interactions at Nucleotide ResolutionbioRxivDecember 18, 2025Iwona Nowak, Ahsan H. Polash, Hang T. Huynh, Mahekdeep Kaur, Vivian Lobo, Jérémy Scutenaire, Michelle Fong, Ghaliah Alluhaibi, Dimitrios G. Anastasakis, Markus Hafner, et al.were generated from 1 μg of total RNA using the NEBNext® rRNA Depletion Kit v2 (New England Biolabs, E7400X) and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, E7760L)

(17) Integration of Short- and Long-Read RNA Sequencing Enables the Discovery of Circular RNAsCancer ResearchDecember 2, 2025Sidi Z. Swift, Jace Webster, Hung Mai, Ghofran Othoum, Li Lin, Amy Ly, Emily B. Rozycki, Jacqueline L. Mudd, Emily Coonrod, Ashna Agarwal, et al.sequencing with the following exceptions. We used the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (#E7400X, New England BioLabs, Ipswich, MA) to remove the rRNA. Per the manufacturer’s protocol, multiple reactions

(18) Viral non-coding RNAs hijack host Pumilio proteins to regulate host transcriptsbioRxivDecember 1, 2025Nhi Phan, Paulina PawlicaNEBNext Ultra II Directional RNA Library Kit (NEB #E7760) with either the rRNA depletion module (NEB #E7400) or the poly(A) mRNA magnetic isolation module (NEB #E7490), according to the manufacturer’s instructions.

(19) Creeping fat-derived mechanosensitive fibroblasts drive intestinal fibrosis in Crohn’s disease stricturesCellNovember 13, 2025Khristian E. Bauer-Rowe, Benjamin Pham, Michelle Griffin, Norah E. Liang, Alexia Kim, John M. Lu, Michael Januszyk, Jason L. Guo, Stefania De Santis, Yue Xing, et al.Sensitivity DNA Kit,Agilent,Cat# 5067-4626 NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat),NEB,Cat# E7400 SG Universal Library Prep Adapter + UDI Primers,SG,"Cat# 700, #110" Trichrome Stain Kit,Abcam,Cat#

(20) Larp1 supports brain growth and spatial memory via post-transcriptional control of the translation machinerybioRxivOctober 14, 2025Mark S. Williams, Maegan J. Watson, Carson C. ThoreencKO mice using RNEasy columns (Qiagen). rRNA was removed using the NEBNext rRNA removal v2 kit (NEB E7400) and paired-end RNA-seq libraries were prepared using the NEBNext Ultra II RNA library prep kit (NEB

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NEBNextrRNA Depletion Kit v2(Human/Mouse/Rat)

NEBNextrRNA Depletion Kit v2(Human/Mouse/Rat) from New England Biolabs

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NEBNextrRNA Depletion Kit v2(Human/Mouse/Rat) from New England Biolabs

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