TSA Fluorescein System, 50-150 slides NEL701A001KT from Akoya Biosciences, a Quanterix company

Product Specs
ItemTSA Fluorescein System, 50-150 slides
CompanyAkoya Biosciences, a Quanterix company
Price $615.02Supplier Page View Company Product Page
Catalog NumberNEL701A001KT
Quantity1
ApplicationsMultiplex IHC
ReactivityHuman, Mouse

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

Company Profile

Website: quanterix.com

(1) B7-H3 and CD39 Co-Localization in Gastric Cancer: A Potential Prognostic Biomarker and Potential Dual-Target for ImmunotherapyTechnology in Cancer Research & TreatmentSeptember 24, 2025Qiange Zhang, Ying Liu, Hanqin Xuan, Shenghua Zhan, Yu Shen, Ruipeng Wang, Siji Chen, Sisi Ding, Cuiping Liu, Lili Huang, et al.temperature. Next, slides were incubated with tyramide signal amplification (TSA) Fluorescein (Cat No: NEL701A001KT, Akoya) for 10 min at room temperature. Between subsequent staining runs, slides were microwaved to

(2) Genetic Variants in KIF7 May Contribute to Supernumerary Tooth FormationInt Dent JAugust 7, 2025Phohathai Chainaphaphorn, Chumpol Ngamphiw, Sissades Tongsima, Kanoknart Chintakanon, Katsushige Kawasaki, Timothy C. Cox, Atsushi Ohazama, James R. Ketudat Cairns, Piranit Nik Kantaputraat 4 °C overnight with an antibody to Kif7 (# MA5-24382, Invitrogen). Tyramide signal amplification (NEL701A001KT, AKOYA Biosciences) was performed to detect the primary antibody. Protein models The structure of

(3) Neural function of Netrin-1 in precancerous lesions of the pancreasNat CommunAugust 2, 2025Hiba Haidar, Anaïs Bellon, Karen Sleiman, Mélanie Hocine, Nicolas Rama, Nicolas Gadot, Darren R. Carpizo, Patrick Mehlen, Fanny Manndetection step was completed using the following dyes: TSA Fluorescein (1:500; AKOYA Biosciences, Cat. #NEL701A001KT) for C1 probes, TSA Cyanine 3 (1:1500; AKOYA Biosciences, Cat. #NEL704A001KT) for C2 probes, and TSA

(4) Phylogenetic variation of layer II cortical immature neurons in dog and horse confirms covariance with brain size and neocortical surfaceBrain Struct FunctJuly 7, 2025Alessia Pattaro, Marco Ghibaudi, Chiara Corrente, Nikita Telitsyn, Jean-Marie Graic, Luca Aresu, Chet C. Sherwood, Luca Bonfantiwashed with PBS 0.01 M, and incubated with Streptavidin-HRP (TSA Fluorescein System (50–150 slides), NEL701A001KT, Akoya Bioscences, Marlborough (MA), USA) for 1 h at RT. The reaction was then detected in an amplification

(5) Neural function of Netrin-1 in precancerous lesions of the pancreasbioRxivMay 22, 2025Hiba Haidar, Anaïs Bellon, Karen Sleiman, Mélanie Hocine, Nicolas Rama, Nicolas Gadot, Darren Carpizo, Patrick Mehlen, Fanny Manndetection step was completed using the following dyes: TSA Fluorescein (1:500; AKOYA Biosciences, Cat. #NEL701A001KT) for C1 probes, TSA Cyanine 3 (1:1500; AKOYA Biosciences, Cat. #NEL704A001KT) for C2 probes, and TSA

(6) Noncanonical Wnt/Ror2 Signaling Regulates Basal Cell Fidelity and Branching Morphogenesis in the Mammary GlandbioRxivFebruary 28, 2025Hongjiang Si, Erika Mendoza Mendoza Mendoza, Madelyn Esquivel, Chad J. Creighton, Jianming Xu, Kevin RoartyImmunodetection Kit,Vector Laboratories,Cat# BMK-2202 TSA Fluorescein System,Akoya Biosciences,Cat# NEL701A001KT RNeasy Mini Kit,Qiagen,Cat# 74104 PicoPure RNA Isolation kit,Thermo Fisher Scientific,Cat# KIT0204 High-Capacity

(7) Zinc finger protein ZC3H18 is abnormally expressed in esophageal cancer tissues and facilitates the proliferation of esophageal cancer cellsFrontiers in ImmunologyFebruary 25, 2025Yujin Zhang, Yilong Wan, Jiaxi Li, Sheng Ju, Xin Tong, Ji Wu, Hao Wu, Liuqing Zhang, Shengxiang Shao, Yuhong Wang, et al.(HRP)-conjugated secondary antibody. Subsequently, Tyramide Signal Amplification (TSA) Fluorescein (Cat No: NEL701A001KT, Akoya) was applied for 10 minutes at room temperature to amplify the signal. Following each staining

(8) Small molecule-mediated inhibition of the oxidoreductase ERO1A restrains aggressive breast cancer by impairing VEGF and PD-L1 in the tumor microenvironmentCell Death DisFebruary 17, 2025Ersilia Varone, Michele Retini, Alessandro Cherubini, Alexander Chernorudskiy, Alice Marrazza, Andrea Guidarelli, Alfredo Cagnotto, Marten Beeg, Marco Gobbi, Stefano Fumagalli, et al.in TBS 0,1 M) for 5 minutes at RT), the slides were incubated in blocking reagent (AKOYA Biosciences NEL701A001KT) for 1 h and 30 minutes at RT. The slides were washed 3 times in TNT for 5 minutes at RT and incubated

(9) Communication between the stem cell niche and an adjacent differentiation niche through miRNA and EGFR signaling orchestrates exit from the stem cell state in the Drosophila ovaryPLOS BiologyMarch 21, 2024Jiani Chen, Chaosqun Li, Yifeng Sheng, Junwei Zhang, Lan Pang, Zhi Dong, Zhiwei Wu, Yueqi Lu, Zhiguo Liu, Qichao Zhang, et al.min. The samples then developed in situ signals using a TSA Fluorescein system (AKOYA Biosciences, Cat#NEL701A001KT). Finally, the ovary samples were mounted in ProLong Gold Antifade Mountant with DAPI (Invitrogen, Cat#P36935),

(10) Feasibility and therapeutical potential of local intracerebral encapsulated cell biodelivery of BDNF to AppNL-G-F knock-in Alzheimer miceAlzheimers Res TherAugust 18, 2023Simone Tambaro, Sumonto Mitra, Ruchi Gera, Bengt Linderoth, Lars U. Wahlberg, Taher Darreh-Shori, Homira Behbahani, Per Nilsson, Maria EriksdotterOn the second day, the positive staining signal was amplified by using TSA Fluorescence System kit (NEL701A001KT, Akoya Biosciences, USA). Briefly, sections were incubated with biotinylated anti-mouse or anti-rabbit

(11) The Expression of the Endocannabinoid Receptors CB2 and GPR55 is Highly Increased during the Progression of Alzheimer’s Disease in AppNL-G-F Knock-In MiceBiology (Basel)May 31, 2023Dina Medina-Vera, Hongjing Zhao, Erika Bereczki, Cristina Rosell-Valle, Makoto Shimozawa, Gefei Chen, Fernando Rodríguez de de Fonseca, Per Nilsson, Simone Tambarowashing step, sections were incubated with Streptavidin–HRP reagent (TSA Fluorescein System Kit, Cat#NEL701A001KT, Akoya Biosciences, Marlborough, MA, USA) diluted 1:100 in TNB blocking buffer for 30 min. Thereafter,

(12) Inducible and Conditional Activation of Adult Neurogenesis Rescues Cadmium-Induced Hippocampus-Dependent Memory Deficits in ApoE4-KI MiceInternational journal of molecular sciencesMay 23, 2023Matsushita MT, Wang H, Abel GM, Xia Z.2 × 30 min in 0.1M borate buffer. For eGFP staining, a tyramide signal amplification kit (TSA; Cat: NEL701A001KT; Akoya Biosciences, Marlborough, MA, USA) was used as previously described [21,25]. 4.9. Imaging and

(13) Stress-induced plasticity of a CRH/GABA projection disrupts reward behaviors in miceNature communicationsFebruary 25, 2023Birnie MT, Short AK, de Carvalho GB, Taniguchi L, Gunn BG, Pham AL, Itoga CA, Xu X, Chen LY, Mahler SV, et al.PerkinElmer, US) for 1.5 h. Fluorescein or cyanine 3-conjugated tyramide was diluted (1:150, Cat #’s NEL701A001KT and NEL704A001KT, respectively) in amplification buffer (Akoya Biosciences, US) and was applied in the

(14) Secondary Degeneration of Oligodendrocyte Precursor Cells Occurs as Early as 24 h after Optic Nerve Injury in RatsInternational journal of molecular sciencesFebruary 9, 2023Toomey LM, Papini MG, Clarke TO, Wright AJ, Denham E, Warnock A, McGonigle T, Bartlett CA, Fitzgerald M, Anyaegbu CC.Kit (1:100, PK-6100, ZG0312, Vector Laboratories) in conjunction with a TSA FLUORESCEIN REAGENT PACK (NEL701A001KT, 191230019, Akoya Biosciences, Marlborough, MA, USA) according to manufacturers’ instructions. To detect

(15) Translational potential of synaptic alterations in Alzheimer's disease patients and amyloid precursor protein knock-in miceBrain communicationsJanuary 1, 2023Medina-Vera D, Enache D, Tambaro S, Abuhashish E, Rosell-Valle C, Winblad B, Rodríguez de Fonseca F, Bereczki E, Nilsson P.washing step, sections were incubated with Streptavidin-HRP reagent (TSA Fluorescein System Kit, Cat#NEL701A001KT, Akoya Biosciences) (1:100) for 30 min. Thereafter, slides were washed in PBST and incubated with fluorophore

(16) Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputsCell reportsDecember 6, 2022Zang Y, Chaudhari K, Bashaw GJ.BP1585100 Tween-20,Bio-Rad,Cat#1706531 Triton X-100,Sigma-Aldrich,Cat#T8787–100ML TSA fluorescein system,AKOYA,Cat#NEL701A001KT Recombinant Mouse Netrin-1 Protein,R & D systems,Cat#1109-N1–025 Schneider’s Drosophila Medium,Life Technologies

(17) Mapping hormone-regulated cell-cell interaction networks in the human breast at single-cell resolutionCell systemsAugust 17, 2022Murrow LM, Weber RJ, Caruso JA, McGinnis CS, Phong K, Gascard P, Rabadam G, Borowsky AD, Desai TA, Thomson M, et al.P4HA1 (1:9000; Thermo PA5-55353), LRRC26 (1:2000; Thermo PA5-63285), FITC-TSA (2 min; Akoya Biosciences NEL701A001KT), Cy3-TSA (3 min; Akoya Biosciences NEL744001KT), Cy5-TSA (7 min; Akoya Biosciences NEL745E001KT). Morphometric

(18) Cell and chromatin transitions in intestinal stem cell regenerationGenes & DevelopmentJune 1, 2022Pratik N.P. Singh, Shariq Madha, Andrew B. Leiter, Ramesh A. Shivdasanitissue sections for 2 h, and tyramide signal amplification with TSA fluorescein (Akoya Biosciences, NEL701A001KT) was used to develop probe channels individually. Slides counterstained with DAPI were imaged with a

(19) Generation of a CRF₁-Cre transgenic rat and the role of central amygdala CRF₁ cells in nociception and anxiety-like behavioreLifeApril 7, 2022Weera MM, Agoglia AE, Douglass E, Jiang Z, Rajamanickam S, Shackett RS, Herman MA, Justice NJ, Gilpin NW.fluorescein TSA reagent, and TSA amplification diluent are part of the TSA detection kit (catalog no. NEL701A001KT, Akoya Biosciences, Marlborough, MA). Sections were washed 3 × 10 min in TNT buffer, mounted on microscope

(20) Lifelong single-cell profiling of cranial neural crest diversification in zebrafishNature communicationsJanuary 1, 0001"Fabian P, Tseng KC, Thiruppathy M, Arata C, Chen HJ, Smeeton J, Nelson N, Crump JG."anti-DIG-HRP (1:500, Perkin Elmer, NEF832001EA) and visualized using Tyr-Fluorescein (1:100, Akoya, NEL701A001KT). The hpdb riboprobe was generated by cloning a purchased gBlock fragment designed from transcript hpdb-201

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TSA Fluorescein System, 50-150 slides from Akoya Biosciences, a Quanterix company

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Akoya Biosciences, a Quanterix company

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TSA Fluorescein System, 50-150 slides from Akoya Biosciences, a Quanterix company

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