Opal 6-Plex Manual Detection Kit (formerly Opal 7-Color Manual IHC Kit) NEL811001KT from Akoya Biosciences, a Quanterix company

Product Specs
ItemOpal 6-Plex Manual Detection Kit (formerly Opal 7-Color Manual IHC Kit)
CompanyAkoya Biosciences, a Quanterix company
Price $1,455.73Supplier Page View Company Product Page
Catalog NumberNEL811001KT
Quantity1
ApplicationsMultiplex IHC
ReactivityHuman

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

Company Profile

Website: quanterix.com

(1) Histone lactylation-mediated up-regulation of IGF2BP2 enhances ferroptosis resistance via Nrf2 in colorectal cancerClin Transl MedDecember 15, 2025Jin-Feng Zhu, Da-Peng Guo, Hai-Na Lv, Zong-Yu Liang, Jing Song, Wei Zengimmunofluorescence, tumour tissue sections of CRC patients were processed using the Opal 7‐Color Manual IHC Kit (NEL811001KT; Akoya Biosciences) with sequential staining for H3K18la (22H2L3, PTM BIO), IGF2BP2 (ab124930; Abcam),

(2) Organ-specific role of neutrophil extracellular traps in shaping the postoperative thrombo-inflammatory niche in pancreatic ductal adenocarcinoma: a multi-model machine learning and mechanistic studyFront ImmunolDecember 9, 2025Songping Cui, Di Wang, Shaocheng Lyu, Jincan Huang, Ren Lang, Jing WangAbcam, dilution 1:3000). For fluorescence detection, we used the opal 3-plex manual detection kit (NEL811001KT, Akoya). We performed dual immunofluorescence staining for MPO and Cit-H3 on tumor tissue sections.

(3) PD-L1 phenotype classification based on expression in tumor and immune cells as a potential biomarker for optimizing anti-PD-1/CTLA-4 immunotherapies in NSCLCJ Immunother CancerDecember 5, 2025Jun Miyakoshi, Tatsuya Yoshida, Yuji Uehara, Yuki Takeyasu, Masayuki Shirasawa, Akito Fukuda, Jumpei Kashima, Shogo Kumagai, Hidehito Horinouchi, Hanako Ono, et al.sections were prepared. Multiplexed fluorescent IHC was performed using the Opal 7-Color Manual IHC Kit (NEL811001KT and NEL861001KT, Akoya Biosciences, Marlborough, Massachusetts, USA) after deparaffinization and heat-induced

(4) SGMS2+ macrophages enhance NR4A3hi NK cell infiltration to improve prognosis and PD-1 treatment efficacy in hepatocellular carcinomaJournal of Translational MedicineNovember 28, 2025Fansheng Meng, Fulin Nian, Haokang Feng, Zhixue Chen, Qiuyu Jiang, Feng Zhang, Lin Xu, Yueying Chen, Hao Zhang, Tao Ye, et al.Opal tyramide signal amplification (TSA) fluorochromes (Opal 7-Color Manual IHC Kit, Perkin Elmer, NEL811001KT) were applied for 20 min at 37 °C. DAPI was applied for visualizing nuclei, and sealed with glycerin.

(5) Machine learning identifies TIME subtypes linking EGFR mutations and immune states in lung adenocarcinomanpj Digital MedicineNovember 26, 2025Zetian Gong, Mingjun Du, Ying Li, Bicheng Ye, Yuming Huang, Hui Gong, Wei Wang, Liang Chen, Zongli Ding, Pengpeng Zhangimmunofluorescence staining was performed on FFPE tissue sections using the Akoya OPAL Polaris 7-Color kit (Akoya, NEL811001KT). After deparaffinization, rehydration in graded alcohols, and antigen retrieval, sections were blocked

(6) TMPRSS11B promotes an acidified microenvironment and immune suppression in squamous lung cancerEMBO ReportsNovember 10, 2025Hari Shankar Sunil, Jean R Clemenceau, Anthony Grichuk, Isabel Barnfather, Sumanth R Nakkireddy, Luke Izzo, Qiang Feng, William Hartnett, Bret M Evers, Lisa Thomas, et al.fluorescent immunohistochemistry was performed using Akoya Opal 3-Plex or 6-plex Detection kit (NEL810001KT, NEL811001KT) following the manufacturer’s instructions. Briefly, FFPE slides were de-paraffinized using xylenes,

(7) Proteomic subtyping highlights tumor heterogeneity of human HCCVirchows ArchivNovember 1, 2025Thomas Ritz, Jovan Tanevski, Jana Baues, Sven H. Loosen, Tom Luedde, Ulf Neumann, Peter Boor, Peter Schirmacher, Julio Saez-Rodriguez, Thomas Longerichperformed using 3-µm tissue sections from the TMA blocks and the Opal multiplex Manual Detection Kit (NEL811001KT, Akoya Biosciences, Marlborough, MA USA). The antibody panel, which was based on the subclassifying

(8) CRISPR/Cas9 library screening reveals that STK19 has synergistic antitumor effects when combined with cisplatin on tongue squamous cell carcinomaJ Transl MedOctober 21, 2025Chunyan Li, Wen Peng, Zhaoming Zhong, Chao Zhang, Xuemin Wang, Rujia Qin, Qian Lei, Jialing Lv, Feineng Liu, Yinghong Zhao, et al.MGMT (Cell Signaling Technology, 1:2000, Cat# 86,039)/Akoya Biosciences supplied a 7-color manual kit (NEL811001KT, 1:800 dilution) including all opal fluorophores and DAPI. The slides were scanned and photographed

(9) SARC028 samples reveal an interplay between TGFβ, interferon signaling and low HLA class I expression as contributors to Ewing sarcoma checkpoint blockade resistanceClinical Cancer ResearchSeptember 2, 2025Jessica D. Daley, Elina Mukherjee, David Ferraro, Shanthi Bhaskar, Anthony Green, Ernest M. Meyer, Hussain Tawbi, Melissa Burgess, Tullia C. Bruno, Anthony R. Cillo, et al.NCL-L-CD20-L26, RRID:AB_563521), using Opal detection fluorophores (Akoya Biosciences, catalog No. NEL811001KT) at the following concentrations: Opal-520 diluted 1:100, Opal-570 diluted 1:150, Opal-540 diluted 1:100,

(10) The role of VISTA engagement in limiting neutrophil-mediated inflammationThe Journal of ImmunologySeptember 1, 2025Elizabeth C Nowak, Jiannan Li, Zachary T Peters, Lindsay K Mendyka, Mohamed A ElTanbouly, Wilson L Davis, Petra Sergent, J Louise Lines, Nicole C Smits, Rodwell Mabaera, et al.Staining was done following the guidelines in the Opal 7-color Manual IHC Kit (Akoya Biosciences, Catalog: NEL811001KT). After the last round of multiplexing, slides are incubated with Spectral DAPI, washed, and mounted

(11) Heterogeneity in Osteosarcoma Revealed by scRNA Sequencing: CLU+ Endothelial Cells as Key Players in Tumor ProgressionCancer ScienceSeptember 1, 2025Zhehao Dai, Peng Xie, Xiangzhi Bai, Tang Liu, Xiaoning Guo, Lei Kuang, Ping Mu, Fangjin Luexperimental protocol. Multi‐IHC staining was conducted using an OPAL 6‐PLEX MANUAL DETECTION kit (NEL811001KT, AKOYA BIOSCIENCES), following the manufacturer's instructions. After primary antibody incubation, the

(12) Elucidating divergent biology in uterine carcinosarcomaTransl OncolAugust 23, 2025Vikas Garg, Stephenie D. Prokopec, Simone C. Stone, Sara Pakbaz, Min Li Chen, Bernard Lam, Czin Czin Benito, Michelle Mcmullen, Ilinca Lungu, Samanta Del Rossi, et al.DAPI; [2] pan-cytokeratin, CD3, CD68, CD163, PD-L1 and DAPI using the OPAL 7-color IHC kit (catalog #: NEL811001KT; Akoya Biosciences, Marlborough, MA, USA) following the manufacturer’s protocol. Sequential staining

(13) ACSS2/AATF Drives Soluble FasL‐Mediated CD8+ T Cell Apoptosis in Pancreatic Neuroendocrine TumorsAdvanced ScienceAugust 12, 2025Qin Dang, Ting Wang, Yan Wang, Zeng Ye, Borui Li, Xuan Pan, Zheng Li, Guixiong Fan, Desheng Jing, Junfeng Xu, et al.performed on formalin‐fixed, paraffin‐embedded sections using the Opal 6‐Plex Manual Detection Kit (NEL811001KT, Akoya Biosciences). Antibody specificity was confirmed by peptide blocking assays.[ 30 ] Optimal

(14) Stratification by Mutational Landscape Reveals Differential Immune Infiltration and Predicts the Recurrence and Clinical Outcome of Cervical CancerPhenomicsAugust 1, 2025Chun Gao, Qian Zhou, Liting Liu, Hong Liu, Yifan Yang, Shen Qu, Qing He, Yafei Huang, Ximiao He, Hui Wangmultiplex immunohistochemistry (mIHC) staining using an opal 7-color manual IHC kit (Akoya biosciences, NEL811001KT), which allows simultaneous illustration of the expression pattern of up to seven antigens. The human

(15) The PAD4 inhibitor GSK484 diminishes neutrophil extracellular trap in the colon mucosa but fails to improve inflammatory biomarkers in experimental colitisBioscience ReportsJune 27, 2025Kangzhe Xie, Jordan Hunter, Aaron Lee, Gulfam Ahmad, Paul K. Witting, Tamara Ortiz-Cerdapresence and density of NETs in colon tissue was visualised by using the Opal 6-Plex Detection Kit (NEL811001KT; AKOYA Biosciences) and simultaneously identifying MPO, CitH3 and NE in the same colon tissues using

(16) Optimization of a thermochemical antibody stripping method for enhanced tyramide signal amplification-based opal multiplex immunohistochemistry in fragile tissuesBMC Res NotesMay 27, 2025Xiaotong Yu, Yan Song, Li Chen, Lixiang Xue, Jianling Yang, Chen Huangsections were deparaffinized and rehydrated according to the Opal 6-Plex Manual Detection Kit protocol (NEL811001KT, Akoya Bioscience). Then, antigen retrieval was performed consistently with the protocol used for IHC

(17) USP2 inhibition unleashes CD47-restrained phagocytosis and enhances anti-tumor immunityNature communicationsMay 16, 2025Dai P, Sun Y, Huang Z, Liu YT, Gao M, Liu HM, Shi J, He C, Xiang B, Yao Y, et al.8.0) using a microwave oven. The mIHC staining was performed using the Opal 7-Color Manual IHC Kit (NEL811001KT, Akoya Biosciences) following the manufacturer’s instructions. After washing with TBST three times,

(18) Circulating immune cells exhibit distinct traits linked to metastatic burden in breast cancerBreast Cancer ResMay 8, 2025S. Mangiola, R. Brown, C. Zhan, J. Berthelet, S. Guleria, C. Liyanage, S. Ostrouska, J. Wilcox, M. Merdas, P. Fuge-Larsen, et al.stained with antibodies using the Opal 6-plex kit for multiplex fluorescent IHC immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol, as previously

(19) Characterizing spatial immune architecture in metastatic melanoma using high-dimensional multiplex imagingFrontiers in ImmunologyApril 29, 2025Joel Eliason, Santhoshi Krishnan, Yasunari Fukuda, Matias A. Bustos, Dan Winkowski, Sungnam Cho, Akshay Basi, Regan Baird, Elizabeth A. Grimm, Michael A. Davies, et al.(mIF) staining was performed using the Akoya Biosciences Opal 7-Color Manual IHC Kit (catalog number NEL811001KT) following the manufacturer’s specifications. Briefly, slides were deparaffinized, rehydrated, and subjected

(20) T-bet+CD8+ T cells govern anti-PD-1 responses in microsatellite-stable gastric cancersNat CommunApril 25, 2025Shiying Tang, Xiaofang Che, Jinyan Wang, Ce Li, Xin He, Kezuo Hou, Xiaojie Zhang, Jia Guo, Bowen Yang, Danni Li, et al.ACRG cohorts. Multiplex immunohistochemistry (mIHC) Opal 7-Color Manual IHC Kit (Akoya Biosciences, NEL811001KT) was used for mIHC. 4 um-thick FFPE sections were dewaxed and rehydrated. In the first round of antigen

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Opal 6-Plex Manual Detection Kit (formerly Opal 7-Color Manual IHC Kit) from Akoya Biosciences, a Quanterix company

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Akoya Biosciences, a Quanterix company

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Opal 6-Plex Manual Detection Kit (formerly Opal 7-Color Manual IHC Kit) from Akoya Biosciences, a Quanterix company

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