Fig 1: Cytokines release induced by DR30318. A Cytokines in PK samples of 0.03 mg/kg group. PK samples were collected at predose, 1 h, 3 h, 7 h and 24 h time points and analyzed for the levels of IL-2 (blue histogram), IL-6 (red histogram), TNF-α (green histogram) and CRP (violet histogram). Data are displayed with Mean ± SD (n = 3). The concentration of IL-2, IL-6 and TNF-α was demonstrated in pg/ml and CRP in μg/ml. B Cytokines in PK samples of 3.0 mg/kg group. PK samples were collected at predose, 1 h, 3 h, 7 h and 24 h time points and analyzed for the levels of IL-2 (blue histogram), IL-6 (red histogram), TNF-α (green histogram) and CRP (violet histogram). Data are displayed with Mean ± SD (n = 3). The concentration of IL-2, IL-6 and TNF-α was demonstrated in pg/ml and CRP in μg/ml. C DR30318 was incubated with PBMC effector cells (n = 2) and CHO-CLDN18.2-gfpx target cells at concentrations of 0, 0.025, 0.5 and 10 μg/ml for 24 h. The red histogram represents the presence of target cells, while the blue histogram represents their absence. The culture supernatant was collected, and the IL-2, IL-6, IL-10, TNF-α and IFN-γ levels were quantified. D DR30318 was incubated with PBMC effector cells (n = 2) and CHO-CLDN18.2-gfpx target cells at 0, 0.025, 0.5 and 10 μg/ml concentrations for 48 h. The red histogram represents the presence of target cells, while the blue histogram represents their absence. The culture supernatant was collected, and the IL-2, IL-6, IL-10, TNF-α and IFN-γ levels were quantified