Fig 1: Cytokine profiles. BALB/C mice (female, aged 8 weeks; n = 3/gp) were immunized with AZD1222 (i.m.; 1.0 × 108 IU) followed by two intranasal boosts with spores (1 × 109 CFU/dose) of WT or SporCoVax (a 1:1 mixture of CotB-RBDWuh and CotC-HR1-HR2Wuh) three and five weeks post prime immunization. Control groups included untreated animals (naïve) and animals primed only (AZD1222). All mice were sacrificed 48 days post prime, and their spleens dissected and stimulated with 2.5 µg/mL rSWuh protein for 72 h, and levels of the cytokines IL-2 (panel (A)), TNF-α (panel (B)) and IL-5 (panel ((C)) determined by flow cytometry.